Extents of Transduction of Human Hematopoietic Cells Recovered From Long-term Engrafted bnxMice
| Experiment No. . | Cell Type . | Transduction . | G418RCFU . | Total hu CFU . | G418 Resistance (%) . |
|---|---|---|---|---|---|
| 1 | CD34+ | 1 hour | 0 | 80 | 0 |
| 1 | 0 | 16 | 0 | ||
| 2 | 0 | 66 | 0 | ||
| 2 | 0 | 21 | 0 | ||
| Average = 0 | 45.8 ± 16.0 | 0% | |||
| 1 | CD34+ | 72 hours | 6 | 37 | 16.2 |
| 1 | 1 | 18 | 5.6 | ||
| 1 | 9 | 53 | 17 | ||
| 2 | 0 | 0 | 0 | ||
| 2 | 5 | 54 | 9.3 | ||
| 2 | 16 | 89 | 18 | ||
| 3 | 8 | 25 | 22.9 | ||
| 3 | 2 | 24 | 8.3 | ||
| 4 | 0 | 15 | 0 | ||
| 4 | 3 | 49 | 6.1 | ||
| Average = 5 ± 1.6 | 36.4 ± 8.1 | 10.3 ± 2.5% | |||
| 1 | CD34+CD38− | 1 hour | 0 | 107 | 0 |
| 1 | 0 | 131 | 0 | ||
| 2 | 0 | 22 | 0 | ||
| 2 | 0 | 59 | 0 | ||
| 2 | 0 | 104 | 0 | ||
| Average = 0 | 84.6 ± 19.5 | 0% | |||
| 1 | CD34+CD38− | 72 hours | 0 | 95 | 0 |
| 1 | 0 | 43 | 0 | ||
| 1 | 0 | 79 | 0 | ||
| 1 | 0 | 9 | 0 | ||
| 2 | 17 | 240 | 7.1 | ||
| 2 | 2 | 211 | 0.9 | ||
| 2 | 0 | 3 | 0 | ||
| 2 | 0 | 10 | 0 | ||
| 3 | 0 | 16 | 0 | ||
| 3 | 0 | 0 | 0 | ||
| 3 | 0 | 25 | 0 | ||
| 4 | 0 | 9 | 0 | ||
| 4 | 0 | 12 | 0 | ||
| 4 | 0 | 26 | 0 | ||
| Average = 1.4 ± 1.2 | 55.6 ± 20.8 | 0.6 ± 0.5% |
| Experiment No. . | Cell Type . | Transduction . | G418RCFU . | Total hu CFU . | G418 Resistance (%) . |
|---|---|---|---|---|---|
| 1 | CD34+ | 1 hour | 0 | 80 | 0 |
| 1 | 0 | 16 | 0 | ||
| 2 | 0 | 66 | 0 | ||
| 2 | 0 | 21 | 0 | ||
| Average = 0 | 45.8 ± 16.0 | 0% | |||
| 1 | CD34+ | 72 hours | 6 | 37 | 16.2 |
| 1 | 1 | 18 | 5.6 | ||
| 1 | 9 | 53 | 17 | ||
| 2 | 0 | 0 | 0 | ||
| 2 | 5 | 54 | 9.3 | ||
| 2 | 16 | 89 | 18 | ||
| 3 | 8 | 25 | 22.9 | ||
| 3 | 2 | 24 | 8.3 | ||
| 4 | 0 | 15 | 0 | ||
| 4 | 3 | 49 | 6.1 | ||
| Average = 5 ± 1.6 | 36.4 ± 8.1 | 10.3 ± 2.5% | |||
| 1 | CD34+CD38− | 1 hour | 0 | 107 | 0 |
| 1 | 0 | 131 | 0 | ||
| 2 | 0 | 22 | 0 | ||
| 2 | 0 | 59 | 0 | ||
| 2 | 0 | 104 | 0 | ||
| Average = 0 | 84.6 ± 19.5 | 0% | |||
| 1 | CD34+CD38− | 72 hours | 0 | 95 | 0 |
| 1 | 0 | 43 | 0 | ||
| 1 | 0 | 79 | 0 | ||
| 1 | 0 | 9 | 0 | ||
| 2 | 17 | 240 | 7.1 | ||
| 2 | 2 | 211 | 0.9 | ||
| 2 | 0 | 3 | 0 | ||
| 2 | 0 | 10 | 0 | ||
| 3 | 0 | 16 | 0 | ||
| 3 | 0 | 0 | 0 | ||
| 3 | 0 | 25 | 0 | ||
| 4 | 0 | 9 | 0 | ||
| 4 | 0 | 12 | 0 | ||
| 4 | 0 | 26 | 0 | ||
| Average = 1.4 ± 1.2 | 55.6 ± 20.8 | 0.6 ± 0.5% |
Bone marrow was recovered from bnx mice after engraftment periods of 9 to 11 months. Human-specific colony-forming assays were plated from the bone marrow of each mouse (3 × 105bnx/hu bone marrow cells per sample) with and without addition of the selective agent G418 (0.9 mg/mL active). The extent of transduction was calculated as: (G418-resistant/total human CFU) × 100 = % G418 resistance.