Human Hematopoietic Lineages Recovered From the Bone Marrow of bnx Mice 9 to 11 Months Post-Transplantation
| Experiment No. . | Transduction Conditions . | Months Engrafted . | hu CD45 (%) . | hu CD4 (%) . | hu CD8 (%) . | hu CD33 (%) . | hu CD19 (%) . |
|---|---|---|---|---|---|---|---|
| 1 | CD34+/1 hour | 9 | 2.2 | 0.6 | 0.6 | 1.0 | 0.0 |
| 1 | 9 | 7.2 | 2.6 | 2.5 | 3.0 | 0.0 | |
| 2 | 11 | 9.3 | 3.1 | 2.0 | 4.1 | 0.0 | |
| 2 | 11 | 4.7 | 1.2 | 1.8 | 1.5 | 0.0 | |
| Average = 5.85 ± 1.5 | 1.88 ± 0.59 | 1.73 ± 0.41 | 2.40 ± 0.71 | 0 ± 0 | |||
| 1 | CD34+/72 hours | 9 | 3.0 | 0.9 | 1.0 | 0.9 | 0.0 |
| 1 | 9 | 2.8 | 0.7 | 1.2 | 1.0 | 0.0 | |
| 1 | 9 | 5.1 | 1.1 | 1.9 | 2.0 | 0.0 | |
| 2 | 11 | 0.1 | 0.0 | 0.0 | 0.0 | 0.0 | |
| 2 | 11 | 3.2 | 1.3 | 0.9 | 1.0 | 0.0 | |
| 2 | 11 | 5.7 | 1.2 | 1.5 | 3.2 | 0.0 | |
| 3 | 10 | 2.9 | 0.8 | 1.0 | 0.9 | 0.0 | |
| 3 | 10 | 1.9 | 0.5 | 0.8 | 0.7 | 0.0 | |
| 4 | 9 | 0.3 | 0.0 | 0.0 | 0.3 | 0.0 | |
| 4 | 9 | 1.5 | 0.4 | 0.6 | 0.5 | 0.0 | |
| Average = 2.65 ± 0.57 | 0.69 ± 0.14 | 0.89 ± 0.19 | 1.05 ± 0.29 | 0 ± 0 | |||
| 1 | CD34+CD38− | 9 | 7.1 | 2.9 | 3.2 | 1.1 | 0.0 |
| 1 | 1 hour | 9 | 4.6 | 1.0 | 2.1 | 1.3 | 0.0 |
| 2 | 11 | 2.4 | 0.8 | 0.9 | 0.7 | 0.0 | |
| 2 | 11 | 5.1 | 2.0 | 1.9 | 1.2 | 0.1 | |
| 2 | 11 | 11.9 | 3.3 | 5.7 | 2.9 | 0.0 | |
| Average = 6.22 ± 1.60 | 2.00 ± 0.50 | 2.76 ± 0.82 | 1.44 ± 0.38 | 0.1 ± .05 | |||
| 1 | CD34+CD38− | 9 | 3.3 | 1.1 | 1.0 | 0.9 | 0.0 |
| 1 | 72 hours | 9 | 4.1 | 1.3 | 1.3 | 1.4 | 0.0 |
| 1 | 9 | 2.6 | 0.7 | 1.0 | 0.9 | 0.0 | |
| 1 | 9 | 3.4 | 1.2 | 0.9 | 1.1 | 0.0 | |
| 2 | 11 | 4.0 | 1.5 | 1.2 | 1.3 | 0.0 | |
| 2 | 11 | 1.5 | 0.3 | 0.7 | 0.4 | 0.0 | |
| 2 | 11 | 11.0 | 2.8 | 3.4 | 4.7 | 0.0 | |
| 2 | 11 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
| 3 | 10 | 6.6 | 1.7 | 2.5 | 2.3 | 0.1 | |
| 3 | 10 | 1.3 | 0.4 | 0.5 | 0.4 | 0.0 | |
| 3 | 10 | 3.6 | 0.8 | 1.3 | 1.1 | 0.0 | |
| 4 | 9 | 0.5 | 0.1 | 0.3 | 0.0 | 0.0 | |
| 4 | 9 | 1.2 | 0.3 | 0.6 | 0.3 | 0.0 | |
| 4 | 9 | 1.8 | 0.4 | 0.4 | 0.7 | 0.0 | |
| Average = 3.21 ± 0.76 | 0.9 ± 0.21 | 1.08 ± 0.24 | 1.11 ± 0.32 | 0.1 ± 0.3 |
| Experiment No. . | Transduction Conditions . | Months Engrafted . | hu CD45 (%) . | hu CD4 (%) . | hu CD8 (%) . | hu CD33 (%) . | hu CD19 (%) . |
|---|---|---|---|---|---|---|---|
| 1 | CD34+/1 hour | 9 | 2.2 | 0.6 | 0.6 | 1.0 | 0.0 |
| 1 | 9 | 7.2 | 2.6 | 2.5 | 3.0 | 0.0 | |
| 2 | 11 | 9.3 | 3.1 | 2.0 | 4.1 | 0.0 | |
| 2 | 11 | 4.7 | 1.2 | 1.8 | 1.5 | 0.0 | |
| Average = 5.85 ± 1.5 | 1.88 ± 0.59 | 1.73 ± 0.41 | 2.40 ± 0.71 | 0 ± 0 | |||
| 1 | CD34+/72 hours | 9 | 3.0 | 0.9 | 1.0 | 0.9 | 0.0 |
| 1 | 9 | 2.8 | 0.7 | 1.2 | 1.0 | 0.0 | |
| 1 | 9 | 5.1 | 1.1 | 1.9 | 2.0 | 0.0 | |
| 2 | 11 | 0.1 | 0.0 | 0.0 | 0.0 | 0.0 | |
| 2 | 11 | 3.2 | 1.3 | 0.9 | 1.0 | 0.0 | |
| 2 | 11 | 5.7 | 1.2 | 1.5 | 3.2 | 0.0 | |
| 3 | 10 | 2.9 | 0.8 | 1.0 | 0.9 | 0.0 | |
| 3 | 10 | 1.9 | 0.5 | 0.8 | 0.7 | 0.0 | |
| 4 | 9 | 0.3 | 0.0 | 0.0 | 0.3 | 0.0 | |
| 4 | 9 | 1.5 | 0.4 | 0.6 | 0.5 | 0.0 | |
| Average = 2.65 ± 0.57 | 0.69 ± 0.14 | 0.89 ± 0.19 | 1.05 ± 0.29 | 0 ± 0 | |||
| 1 | CD34+CD38− | 9 | 7.1 | 2.9 | 3.2 | 1.1 | 0.0 |
| 1 | 1 hour | 9 | 4.6 | 1.0 | 2.1 | 1.3 | 0.0 |
| 2 | 11 | 2.4 | 0.8 | 0.9 | 0.7 | 0.0 | |
| 2 | 11 | 5.1 | 2.0 | 1.9 | 1.2 | 0.1 | |
| 2 | 11 | 11.9 | 3.3 | 5.7 | 2.9 | 0.0 | |
| Average = 6.22 ± 1.60 | 2.00 ± 0.50 | 2.76 ± 0.82 | 1.44 ± 0.38 | 0.1 ± .05 | |||
| 1 | CD34+CD38− | 9 | 3.3 | 1.1 | 1.0 | 0.9 | 0.0 |
| 1 | 72 hours | 9 | 4.1 | 1.3 | 1.3 | 1.4 | 0.0 |
| 1 | 9 | 2.6 | 0.7 | 1.0 | 0.9 | 0.0 | |
| 1 | 9 | 3.4 | 1.2 | 0.9 | 1.1 | 0.0 | |
| 2 | 11 | 4.0 | 1.5 | 1.2 | 1.3 | 0.0 | |
| 2 | 11 | 1.5 | 0.3 | 0.7 | 0.4 | 0.0 | |
| 2 | 11 | 11.0 | 2.8 | 3.4 | 4.7 | 0.0 | |
| 2 | 11 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
| 3 | 10 | 6.6 | 1.7 | 2.5 | 2.3 | 0.1 | |
| 3 | 10 | 1.3 | 0.4 | 0.5 | 0.4 | 0.0 | |
| 3 | 10 | 3.6 | 0.8 | 1.3 | 1.1 | 0.0 | |
| 4 | 9 | 0.5 | 0.1 | 0.3 | 0.0 | 0.0 | |
| 4 | 9 | 1.2 | 0.3 | 0.6 | 0.3 | 0.0 | |
| 4 | 9 | 1.8 | 0.4 | 0.4 | 0.7 | 0.0 | |
| Average = 3.21 ± 0.76 | 0.9 ± 0.21 | 1.08 ± 0.24 | 1.11 ± 0.32 | 0.1 ± 0.3 |
Bone marrow was recovered from bnx mice 9-11 months after cotransplantation of transduced human hematopoietic cells and IL-3 producing stromal cells. The percentage of human CD45+cells in the marrow (BM) was determined by FACS. The percentages of human cell that were positive for each lineage marker by FACS analysis of the ungated bone marrow cells are shown. In each experiment, normal human peripheral blood and age-matched, nontransplanted bnxmice were analyzed concurrently to verify the species-specificity of the monoclonal antibodies.