Replacement and Silent Mutations in VH Segments of Rearranged Ig Genes of CD5+ DLBL
| Case No. . | Germline Usage . | FR I . | CDR I . | FR II . | CDR II . | FR III . | R/S Ratio . | P (FRs) . | P (CDRs) . | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Expected . | Observed . | Expected . | Observed . | Expected . | Observed . | Expected . | Observed . | Expected . | Observed . | ||||||||||||||||
| R . | S . | R . | S . | R . | S . | R . | S . | R . | S . | R . | S . | R . | S . | R . | S . | R . | S . | R . | S . | FRs . | CDRs . | ||||
| 1 | VH3-7 | 6 | 2 | 2 | 6 | 1 | 0 | 6 | 0 | 3 | 1 | 0 | 0 | 3 | 1 | 3 | 1 | 6 | 2 | 3 | 4 | 0.5 | 9 | 1.09 × 10−4 | .01 |
| 2 | VH4-34 | 9 | 3 | 2 | 4 | 1 | 1 | 4 | 1 | 4 | 1 | 4 | 2 | 5 | 2 | 8 | 1 | 9 | 3 | 8 | 4 | 1.4 | 6 | 3.70 × 10−3 | 8.59 × 10−3 |
| 6 | VH5-51 | 7 | 2 | 12 | 1 | 1 | 1 | 1 | 1 | 3 | 1 | 0 | 1 | 4 | 1 | 6 | 0 | 7 | 3 | 6 | 2 | 4.5 | 7 | .14 | .11 |
| 11 | VH4-34 | 3 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 6 | 0 | 3 | 1 | 5 | 0 | 5 | Inf | .05 | 4.77 × 10−4 |
| Case No. . | Germline Usage . | FR I . | CDR I . | FR II . | CDR II . | FR III . | R/S Ratio . | P (FRs) . | P (CDRs) . | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Expected . | Observed . | Expected . | Observed . | Expected . | Observed . | Expected . | Observed . | Expected . | Observed . | ||||||||||||||||
| R . | S . | R . | S . | R . | S . | R . | S . | R . | S . | R . | S . | R . | S . | R . | S . | R . | S . | R . | S . | FRs . | CDRs . | ||||
| 1 | VH3-7 | 6 | 2 | 2 | 6 | 1 | 0 | 6 | 0 | 3 | 1 | 0 | 0 | 3 | 1 | 3 | 1 | 6 | 2 | 3 | 4 | 0.5 | 9 | 1.09 × 10−4 | .01 |
| 2 | VH4-34 | 9 | 3 | 2 | 4 | 1 | 1 | 4 | 1 | 4 | 1 | 4 | 2 | 5 | 2 | 8 | 1 | 9 | 3 | 8 | 4 | 1.4 | 6 | 3.70 × 10−3 | 8.59 × 10−3 |
| 6 | VH5-51 | 7 | 2 | 12 | 1 | 1 | 1 | 1 | 1 | 3 | 1 | 0 | 1 | 4 | 1 | 6 | 0 | 7 | 3 | 6 | 2 | 4.5 | 7 | .14 | .11 |
| 11 | VH4-34 | 3 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 6 | 0 | 3 | 1 | 5 | 0 | 5 | Inf | .05 | 4.77 × 10−4 |
Abbreviations: FR, framework region; CDR, complementarity determining region; R, replacement mutation; S, silent mutation;P, probability excess or scarcity of replacement mutations resulted from chance only; Inf, infinite.