Comparison of Leptin With G-CSF, IL-3, GM-CSF, and SCF on the Effects of Colony Formation From BMC of Normal Mice
| Factors . | No. of Colonies per 2.5 × 104 Cells . | |||||||
|---|---|---|---|---|---|---|---|---|
| . | GM . | E . | Mk . | EM . | GMM . | GEM . | GEMM . | Total . |
| Leptin | 31 ± 4 | 0 | 0 | 0 | 0 | 0 | 0 | 31 ± 4 |
| G-CSF | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Leptin + G-CSF | 24 ± 3 | 0 | 0 | 0 | 0 | 0 | 0 | 24 ± 3 |
| IL-3 | 58 ± 5 | 3 ± 2 | 3 ± 2 | 2 ± 1 | 1 ± 2 | 11 ± 6 | 3 ± 3 | 81 ± 7 |
| Leptin + IL-3 | 60 ± 3 | 4 ± 3 | 2 ± 2 | 0 | 2 ± 1 | 21 ± 4 | 3 ± 2 | 93 ± 6 |
| GM-CSF | 26 ± 1 | 0 | 0 | 0 | 0 | 0 | 0 | 26 ± 1 |
| Leptin + GM-CSF | 26 ± 2 | 0 | 0 | 0 | 0 | 0 | 0 | 26 ± 2 |
| SCF | 35 ± 8 | 26 ± 5 | 0 | 0 | 0 | 1 ± 1 | 0 | 62 ± 10 |
| Leptin + SCF | 74 ± 9* | 25 ± 4 | 0 | 1 ± 1 | 0 | 20 ± 2* | 2 ± 1 | 121 ± 12* |
| Factors . | No. of Colonies per 2.5 × 104 Cells . | |||||||
|---|---|---|---|---|---|---|---|---|
| . | GM . | E . | Mk . | EM . | GMM . | GEM . | GEMM . | Total . |
| Leptin | 31 ± 4 | 0 | 0 | 0 | 0 | 0 | 0 | 31 ± 4 |
| G-CSF | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Leptin + G-CSF | 24 ± 3 | 0 | 0 | 0 | 0 | 0 | 0 | 24 ± 3 |
| IL-3 | 58 ± 5 | 3 ± 2 | 3 ± 2 | 2 ± 1 | 1 ± 2 | 11 ± 6 | 3 ± 3 | 81 ± 7 |
| Leptin + IL-3 | 60 ± 3 | 4 ± 3 | 2 ± 2 | 0 | 2 ± 1 | 21 ± 4 | 3 ± 2 | 93 ± 6 |
| GM-CSF | 26 ± 1 | 0 | 0 | 0 | 0 | 0 | 0 | 26 ± 1 |
| Leptin + GM-CSF | 26 ± 2 | 0 | 0 | 0 | 0 | 0 | 0 | 26 ± 2 |
| SCF | 35 ± 8 | 26 ± 5 | 0 | 0 | 0 | 1 ± 1 | 0 | 62 ± 10 |
| Leptin + SCF | 74 ± 9* | 25 ± 4 | 0 | 1 ± 1 | 0 | 20 ± 2* | 2 ± 1 | 121 ± 12* |
A total of 2.5 × 104 BMC were plated per dish in the presence of 2U/mL Epo and designated factors. The number of colonies indicates mean ± SD of triplicate cultures.
Significantly different from their counterparts without leptin (P < .05).