Expression of the Exon-6–Deleted COX cDNA in E coli
| . | Construct . | COX Activity* . |
|---|---|---|
| Normal control | pGEX-2T:COX/sense | 15,870 |
| Negative control | pGEX-2T:COX/antisense | 1,317 |
| Exon-6–deleted cDNA | pGEX-2T:COX/del. exon 6 | 629 |
| . | Construct . | COX Activity* . |
|---|---|---|
| Normal control | pGEX-2T:COX/sense | 15,870 |
| Negative control | pGEX-2T:COX/antisense | 1,317 |
| Exon-6–deleted cDNA | pGEX-2T:COX/del. exon 6 | 629 |
Expression vector: pGEX-2T. Values are expressed as the mean of two duplicate experiments.
Activity given as picomoles of protoporphyrin per hour per milligram at 37°C.