Inhibition of CD34 MoAb-Induced Homotypic Cell Adhesion by MoAbs to Other Adhesion Molecules
| MoAbs . | Adhesion Score . |
|---|---|
| None | +++ |
| Negative control | +++ |
| CD11a (M17/4) | ++ |
| CD11a (2D7) | ++ |
| CD11b (M1/70) | +++ |
| CD11c (HL3) | +++ |
| CD18 (C71/16) | ++ |
| CD18 (M18/2) | + |
| CD29 (9EG7) | +++ |
| CD31 (MEC13.3) | +++ |
| CD44 (1M7) | +++ |
| CD45 (30-F11) | +++ |
| CD45R (RA3-6B2) | +++ |
| CD49d (R1-2) | +++ |
| CD54 (3E2) | ++ |
| CD54 (3E2B) | ++ |
| CD54 (KAT-1) | + |
| CD62L (MEL-14) | +++ |
| CD71 (C2) | +++ |
| Integrin β7 | +++ |
| MoAbs . | Adhesion Score . |
|---|---|
| None | +++ |
| Negative control | +++ |
| CD11a (M17/4) | ++ |
| CD11a (2D7) | ++ |
| CD11b (M1/70) | +++ |
| CD11c (HL3) | +++ |
| CD18 (C71/16) | ++ |
| CD18 (M18/2) | + |
| CD29 (9EG7) | +++ |
| CD31 (MEC13.3) | +++ |
| CD44 (1M7) | +++ |
| CD45 (30-F11) | +++ |
| CD45R (RA3-6B2) | +++ |
| CD49d (R1-2) | +++ |
| CD54 (3E2) | ++ |
| CD54 (3E2B) | ++ |
| CD54 (KAT-1) | + |
| CD62L (MEL-14) | +++ |
| CD71 (C2) | +++ |
| Integrin β7 | +++ |
BaF3-CD34 cells were preincubated with the respective MoAbs (final concentration, 50 μg/mL) for 30 minutes at 0°C before initiation of the homotypic cell adhesion assay by adding anti-CD34 MoAb QBEND 10 (final concentration, 5 μg/mL). Cell aggregation was scored after 90 minutes. Identical results were obtained in three independent experiments.