BaF3-CD34 cells were preincubated with inhibitors for 30 minutes at 37°C before initiation of the homotypic cell adhesion assay by adding anti-CD34 MoAb QBEND 10 (final concentration, 5 μg/mL). Inhibitors were used as follows: 30 mmol/L sodium fluoride, 5 mmol/L EDTA, 10 μg/mL cytochalasin B, 1 μg/mL actinomycin D, 1 μg/mL cycloheximide, 0.02% sodium azide, 5 μmol/L herbimycin A, 1 μmol/L staurosporine, 100 μmol/L H7. Cell aggregation was scored after 90 minutes. Identical results were obtained in three independent experiments.