Effect of PMA, fMLP, and GM-CSF on Kinetics of [3H]-2-DOG Uptake by Human Peripheral Blood Neutrophils
| Cell Treatment . | No. . | Km (mmol/L) . | Vmax (nmol/106 cells/min) . |
|---|---|---|---|
| None | 4 | 0.99 ± 0.03 | 2.91 ± 0.09 |
| PMA (10 min) | 4 | 0.61 ± 0.08* | 2.78 ± 0.19 |
| PMA (30 min) | 2 | 0.47 ± 0.01† | 2.99 ± 0.02 |
| fMLP (10 min) | 3 | 0.20 ± 0.02† | 1.65 ± 0.20 |
| GM-CSF (60 min) | 3 | 0.22 ± 0.04† | 1.40 ± 0.33 |
| Cell Treatment . | No. . | Km (mmol/L) . | Vmax (nmol/106 cells/min) . |
|---|---|---|---|
| None | 4 | 0.99 ± 0.03 | 2.91 ± 0.09 |
| PMA (10 min) | 4 | 0.61 ± 0.08* | 2.78 ± 0.19 |
| PMA (30 min) | 2 | 0.47 ± 0.01† | 2.99 ± 0.02 |
| fMLP (10 min) | 3 | 0.20 ± 0.02† | 1.65 ± 0.20 |
| GM-CSF (60 min) | 3 | 0.22 ± 0.04† | 1.40 ± 0.33 |
Peripheral blood neutrophils were stimulated with PMA (100 ng/mL), fMLP (0.5 μmol/L), or GM-CSF (100 ng/mL) for the times indicated before measuring [3H]-2-DOG uptake at increasing 2-DOG concentrations (0.1 to 4.0 mmol/L). Data were analyzed using Lineweaver Burk plots. Values are the mean ± SEM of the indicated number of experiments involving duplicate or triplicate determinations.
Significantly different from control, P < .01.
P < .002.