Table 1.

Percentage of Spontaneous and F-ara-A–Induced Apoptosis in Antigen-Defined Peripheral Lymphocytes in CLL Patients and Bcl-2 Protein Expression

Patient No. Rai StageSpontaneous Apoptosis (%)F-ara-A–Induced Apoptosis (%) Bcl-2 (ABC)
CD19+CD3+CD19+CD3+
1  0  6.9  9.0  85.8  61.49  48.756  
2  13.2  3.8  52.2  35.1  31.726  
3  0  30.6 5.8  54.5  34.3  33.150  
4  0  43.8  1.8 81.6  35.3  28.258  
5  0  1.2  5.5  74.7 29.4  34.728  
6  0  7.5  8.6  82.8  58.9 76.320  
7  0  1.1  0.9  41.2  53.8  41.109 
8  0  20.3  8.3  21.8  30.2  19.644  
9  4.7  10.1  13.1  43.0  ND  
10  0  25.4 8.3  60.3  17.7  28.380  
11  0  6.2  4.4 66.4  63.8  68.688  
12  I  38.2  6.3  81.2 56.6  60.054  
13  I  39.1  3.2  97.9  39.1 34.464  
14  I  14.1  16.1  33.2  52.5 ND  
15  I  7.6  4.6  40.0  20.0 ND  
16  I  12.8  7.5  58.9  16.1 20.814  
17  I  7.2  11.2  36.1  46.4  25.426 
18  I  17.5  16.6  68.0  58.2  ND  
19 II  5.17  2.2  28.8  22.2  48.858  
20  III 9.8  9.9  28.9  86.5  57.084  
21  III  3.3 17.7  3.9  82.4  20.808  
22  III  6.2  18.8 19.5  90.2  38.814  
23  IV  10.6  7.0  16.7 67.4  100.200  
24  IV  6.5  16.2  11.9  62.2 ND  
25  IV  14.9  12.1  75.6  57.8 45.726 
Patient No. Rai StageSpontaneous Apoptosis (%)F-ara-A–Induced Apoptosis (%) Bcl-2 (ABC)
CD19+CD3+CD19+CD3+
1  0  6.9  9.0  85.8  61.49  48.756  
2  13.2  3.8  52.2  35.1  31.726  
3  0  30.6 5.8  54.5  34.3  33.150  
4  0  43.8  1.8 81.6  35.3  28.258  
5  0  1.2  5.5  74.7 29.4  34.728  
6  0  7.5  8.6  82.8  58.9 76.320  
7  0  1.1  0.9  41.2  53.8  41.109 
8  0  20.3  8.3  21.8  30.2  19.644  
9  4.7  10.1  13.1  43.0  ND  
10  0  25.4 8.3  60.3  17.7  28.380  
11  0  6.2  4.4 66.4  63.8  68.688  
12  I  38.2  6.3  81.2 56.6  60.054  
13  I  39.1  3.2  97.9  39.1 34.464  
14  I  14.1  16.1  33.2  52.5 ND  
15  I  7.6  4.6  40.0  20.0 ND  
16  I  12.8  7.5  58.9  16.1 20.814  
17  I  7.2  11.2  36.1  46.4  25.426 
18  I  17.5  16.6  68.0  58.2  ND  
19 II  5.17  2.2  28.8  22.2  48.858  
20  III 9.8  9.9  28.9  86.5  57.084  
21  III  3.3 17.7  3.9  82.4  20.808  
22  III  6.2  18.8 19.5  90.2  38.814  
23  IV  10.6  7.0  16.7 67.4  100.200  
24  IV  6.5  16.2  11.9  62.2 ND  
25  IV  14.9  12.1  75.6  57.8 45.726 

Peripheral lymphocytes from 25 CLL patients were incubated alone or with F-ara-A, 3 μmol/L for 72 hours. Apoptosis was assessed as the percentage of ISEL-positive cells within the CD3+ and the CD19+ antigen-defined population. Bcl-2 protein expression was quantified in CD5/CD19+ lymphocytes as described in Materials and Methods.

Abbreviation: ND, not determined.