Effect of Protease and Neuraminidase Treatment on HL-60 Adhesion to L-Selectin and P-Selectin Under Flow Conditions
| Treatment . | Percent of Control Adhesion . | |
|---|---|---|
| L-Selectin . | P-Selectin . | |
| O-sialoglycoprotein endopeptidase | 0 ± 0 | 3.7 ± 3.7 |
| Chymotrypsin | 0 ± 0 | 0 ± 0 |
| Neuraminidase, V cholerae | 7.0 ± 7.0 | 70.6 ± 5.5 |
| Treatment . | Percent of Control Adhesion . | |
|---|---|---|
| L-Selectin . | P-Selectin . | |
| O-sialoglycoprotein endopeptidase | 0 ± 0 | 3.7 ± 3.7 |
| Chymotrypsin | 0 ± 0 | 0 ± 0 |
| Neuraminidase, V cholerae | 7.0 ± 7.0 | 70.6 ± 5.5 |
HL-60 cells were treated with enzymes as described in Materials and Methods and were perfused (0.5 × 106 cells/mL) through the flow chamber at a wall shear stress of 1 dyne/cm2 in which human L-selectin–IgG (2 μg/mL), P-selectin–IgG (2 μg/mL), or purified P-selectin (1:60) were adsorbed to the lower wall of the chamber. Control binding for HL-60 cells on L-selectin–IgG, P-selectin–IgG, and purified P-selectin was 43 ± 3, 218 ± 24, and 68 ± 8 cells/mm2, respectively. Mean ± SEM percent of control binding/mm2 in multiple fields from two to three independent experiments.