FRET Technique Can Detect c-kit Receptor Dimerization in Progeny of Normal Human BFU-E
| . | Growth Factor . | FITC Mean Fluorescence Intensity . | Cy3 Mean Fluorescence Intensity . | Acceptor/ Donor Ratio . |
|---|---|---|---|---|
| Experiment 1A | 0 | 16.7 | 17.4 | 1.04 |
| SCF | 7.8 | 26.7 | 3.42 | |
| Experiment 1B | 0 | 16.8 | 18.2 | 1.08 |
| SCF | 8.4 | 25.6 | 3.04 |
| . | Growth Factor . | FITC Mean Fluorescence Intensity . | Cy3 Mean Fluorescence Intensity . | Acceptor/ Donor Ratio . |
|---|---|---|---|---|
| Experiment 1A | 0 | 16.7 | 17.4 | 1.04 |
| SCF | 7.8 | 26.7 | 3.42 | |
| Experiment 1B | 0 | 16.8 | 18.2 | 1.08 |
| SCF | 8.4 | 25.6 | 3.04 |
Normal human peripheral blood BFU-E progeny were plucked on day 10 of culture, pooled, and incubated for 3 hours at 37°C in the absence of SCF to permit internalization and degradation of SCF acquired during culture.33 34 The BFU-E progeny were then aliquoted into individual test tubes for experiment 1A or experiment 1B, labeled with 104D2-FITC plus 104D2-Cy3 at 4°C, incubated without or with SCF (200 ng/mL) for 1 hour at 37°C, fixed, and analyzed by flow cytometry as described in Table 2.