Monoclonal Antibody 104D2 Does not Block Binding of SCF to the c-kit Receptor
| Addition . | cpm Bound . |
|---|---|
| 125I-SCF | 1,050 ± 39 |
| 125I-SCF + unlabeled SCF | 177 ± 22 |
| 125I-SCF + 104D2 | 1,042 ± 22 |
| Addition . | cpm Bound . |
|---|---|
| 125I-SCF | 1,050 ± 39 |
| 125I-SCF + unlabeled SCF | 177 ± 22 |
| 125I-SCF + 104D2 | 1,042 ± 22 |
OCIM1 cells were incubated with 125I-SCF (800 pmol/L) without or with unlabeled SCF (80 nmol/L) or with 104D2 monoclonal antibody (200 μg/mL) for 1 hour at 37°C as previously described.38 The data are presented as the mean (±SEM) of triplicate values.