Cytokines Produced by Parental or IL-2 Gene–Transduced NK Cell Lines
| Cell Line . | TNFα . | IFNγ . | ||
|---|---|---|---|---|
| Experiment 1 . | Experiment 2 . | Experiment 1 . | Experiment 2 . | |
| P-NK-92 (IL-2+)* | 20 | 0 | 218 | 1,000 |
| P-NK-92 (IL-2−)† | 0 | 0 | 0 | 0 |
| TR-IL-2-NK-92‡ | 20 | 0 | 41 | 400 |
| P-YT | 0 | 0 | 500 | 288 |
| TR-IL-2-YT | 0 | 0 | 238 | 225 |
| Cell Line . | TNFα . | IFNγ . | ||
|---|---|---|---|---|
| Experiment 1 . | Experiment 2 . | Experiment 1 . | Experiment 2 . | |
| P-NK-92 (IL-2+)* | 20 | 0 | 218 | 1,000 |
| P-NK-92 (IL-2−)† | 0 | 0 | 0 | 0 |
| TR-IL-2-NK-92‡ | 20 | 0 | 41 | 400 |
| P-YT | 0 | 0 | 500 | 288 |
| TR-IL-2-YT | 0 | 0 | 238 | 225 |
The data from 2 independent experiments are expressed as pg/106 cells measured in 48-hour supernatants. Cultures of NK cells were evaluated 6 to 8 weeks after selection.
Parental NK-92 cells incubated with 6,000 IU/mL IL-2.
Parental NK-92 cells incubated in the absence of IL-2.
IL-2 gene–transduced NK-92 cells incubated in the absence of IL-2.