Th1 and Th2 Cytokine Profile in Supernatants From PBMC, Cultured Lesional, and Uninvolved Skin From C-KS and AIDS-KS Patients and From PBMC of Patients With Dermatologic Diseases Other Than KS
. | IL-4 (pg/mL) . | γIFN (pg/mL) . |
---|---|---|
C-KS | ||
PBMC (n = 18) | 0 | 3,775 ± 638 |
Lesional skin (−CM) (n = 9) | 0 | 0 |
Lesional skin (+CM) (n = 9) | 6.5 ± 2.0 | 1,005 ± 369 |
Uninvolved skin (−CM) (n = 3) | 0 | 0 |
Uninvolved skin (+CM) (n = 3) | 0 | 0 |
AIDS-KS | ||
PBMC (n = 22) | 191 ± 300 | 4,658 ± 3,350 |
Lesional skin (−CM) (n = 9) | 0 | 0 |
Lesional skin (+CM) (n = 9) | 59 ± 44 | 1,016 ± 721 |
Other dermatologic diseases (n = 34) | ||
PBMC (−CM) | 0 | 4 ± 1.4 |
PBMC (+CM) | 0 | 600 ± 196 |
. | IL-4 (pg/mL) . | γIFN (pg/mL) . |
---|---|---|
C-KS | ||
PBMC (n = 18) | 0 | 3,775 ± 638 |
Lesional skin (−CM) (n = 9) | 0 | 0 |
Lesional skin (+CM) (n = 9) | 6.5 ± 2.0 | 1,005 ± 369 |
Uninvolved skin (−CM) (n = 3) | 0 | 0 |
Uninvolved skin (+CM) (n = 3) | 0 | 0 |
AIDS-KS | ||
PBMC (n = 22) | 191 ± 300 | 4,658 ± 3,350 |
Lesional skin (−CM) (n = 9) | 0 | 0 |
Lesional skin (+CM) (n = 9) | 59 ± 44 | 1,016 ± 721 |
Other dermatologic diseases (n = 34) | ||
PBMC (−CM) | 0 | 4 ± 1.4 |
PBMC (+CM) | 0 | 600 ± 196 |
Cytokine profile from KS patients and patients with dermatologic diseases other than KS (10 psoriatic patients and 24 with chronic dermatitis). PBMC were isolated, plated at a concentration of 106 cells/mL and stimulated with 1 μg/mL final dilution of PHA for 3 days to assess cytokine production. Lesional and uninvolved skin were cultured in the presence or absence of TCM, as described in Materials and Methods. The supernatants from these cultures were then assessed for cytokine levels. The results are given as M ± SD of the values (pg/mL) and are devoid of the levels present in TCM.