Myeloid Progenitor Assays of C57BL6/J-hbd/hbdand C57BL6/J-+hbd/+hbd Marrow
| . | C57BL6/J-+/+ (n = 8) . | C57BL6/J-hbd/hbd (n = 8) . |
|---|---|---|
| CFU-GM | 54.8 ± 2.1 | 100.5 ± 4.0 |
| BFU-E | 4.6 ± 0.84 | 7.5 ± 0.96 |
| CFU-GEMM | 1.13 ± 0.64 | 2.13 ± 0.74 |
| CFU-S | 11.71 ± 0.73 (n = 14) | 15.42 ± 0.76 (n = 14) |
| . | C57BL6/J-+/+ (n = 8) . | C57BL6/J-hbd/hbd (n = 8) . |
|---|---|---|
| CFU-GM | 54.8 ± 2.1 | 100.5 ± 4.0 |
| BFU-E | 4.6 ± 0.84 | 7.5 ± 0.96 |
| CFU-GEMM | 1.13 ± 0.64 | 2.13 ± 0.74 |
| CFU-S | 11.71 ± 0.73 (n = 14) | 15.42 ± 0.76 (n = 14) |
BM cells (4.5 × 104) from either a C57BL6/J-+hbd/+hbd or C57BL6/J-hbd/hbd mouse were grown in methylcellulose media containing recombinant growth factors. Three colony types were scored: CFU-GM, CFU-GEMM, and BFU-E. Each value represents the mean number of colonies per 4.5 × 104 BM cells plated from two combined experiments ± SE. P < .05 for all comparisons except CFU-GEMM where P = .32. For CFU-S, either C57BL6/J-hbd/hbd and C57BL6/J-+hbd/+hbd marrow was injected into irradiated C57BL6/J-+hbd/+hbd mice. Ten days postinjection spleen colonies were counted. The means are based on two independent experiments. For the CFU-S comparison, P = .0016.