Analysis of BV Subfamily Frequencies Under SEA Stimulation of Adult and CB Lymphocytes
| BV . | Adult PBL (%) . | CB PBL (%) . | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| n°1 . | n°2 . | n°3 . | n°4 . | n°1 . | n°2 . | n°3 . | n°4 . | |||||||||
| SEA . | Medium . | SEA . | Medium . | SEA . | Medium . | SEA . | Medium . | SEA . | Medium . | SEA . | Medium . | SEA . | Medium . | SEA . | Medium . | |
| 1 | 1.0 | 2.9 | 1.3 | 2.8 | 0.8 | 4.0 | 2.7 | 2.3 | 1.0 | 2.2 | 2.1 | 4.1 | 1.8 | 3.6 | 3.5 | 1.3 |
| 5 | 5.1 | 3.6 | 3.5 | 4.8 | 6.9* | 2.7 | 4.2 | 8.8 | 6.5 | 5.8 | 6.4 | 7.0 | 19.5 | 3 | 22 | 4.2 |
| 6 | 31.2 | 20.8 | 21.3 | 11.2 | 30.6 | 19.3 | 22.4 | 13.9 | 27.4 | 16.2 | 24.4 | 8.0 | 1 | 14.4 | 40.2 | 14.3 |
| 7 | 18.2 | 4.3 | 16.5 | 7.6 | 0.3 | 0.3 | <0.1 | <0.1 | 10.9 | 3.0 | <0.1 | 0.3 | <0.1 | 1.4 | <0.1 | 4.1 |
| 9 | 4.3 | 5.3 | 5.4 | 4.6 | 6.1 | 4.8 | 4.4 | 5.4 | 7.5 | 6.4 | 7.2 | 2.9 | 7.8 | <0.1 | <0.1 | <0.1 |
| 18 | 6.6 | 1.7 | 7.6 | 3.5 | 3.8 | <0.1 | 0.1 | <0.1 | 9.2 | 1.3 | 1.2 | <0.1 | 2.1 | <0.1 | 8.5 | <0.1 |
| 21 | 5.1 | 2.4 | 3.5 | 4.0 | 8.8 | 5.5 | 9.4 | <0.1 | 4.2 | 2.8 | 5.8 | 10.0 | 5.1 | 4.8 | 5.1 | 7.2 |
| 22 | 17.3 | 14.2 | 16.4 | 6.7 | 22.3 | 6.0 | 17.8 | <0.1 | 21.5 | 11.0 | 20.7 | 6.0 | 14.5 | 5.7 | 17.8 | 9.5 |
| 24 | 2.2 | <0.1 | 2.2 | <0.1 | 0.3 | <0.1 | 3.0 | <0.1 | 1.4 | 0.8 | 0.2 | <0.1 | <0.1 | <0.1 | <0.1 | <0.1 |
| BV . | Adult PBL (%) . | CB PBL (%) . | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| n°1 . | n°2 . | n°3 . | n°4 . | n°1 . | n°2 . | n°3 . | n°4 . | |||||||||
| SEA . | Medium . | SEA . | Medium . | SEA . | Medium . | SEA . | Medium . | SEA . | Medium . | SEA . | Medium . | SEA . | Medium . | SEA . | Medium . | |
| 1 | 1.0 | 2.9 | 1.3 | 2.8 | 0.8 | 4.0 | 2.7 | 2.3 | 1.0 | 2.2 | 2.1 | 4.1 | 1.8 | 3.6 | 3.5 | 1.3 |
| 5 | 5.1 | 3.6 | 3.5 | 4.8 | 6.9* | 2.7 | 4.2 | 8.8 | 6.5 | 5.8 | 6.4 | 7.0 | 19.5 | 3 | 22 | 4.2 |
| 6 | 31.2 | 20.8 | 21.3 | 11.2 | 30.6 | 19.3 | 22.4 | 13.9 | 27.4 | 16.2 | 24.4 | 8.0 | 1 | 14.4 | 40.2 | 14.3 |
| 7 | 18.2 | 4.3 | 16.5 | 7.6 | 0.3 | 0.3 | <0.1 | <0.1 | 10.9 | 3.0 | <0.1 | 0.3 | <0.1 | 1.4 | <0.1 | 4.1 |
| 9 | 4.3 | 5.3 | 5.4 | 4.6 | 6.1 | 4.8 | 4.4 | 5.4 | 7.5 | 6.4 | 7.2 | 2.9 | 7.8 | <0.1 | <0.1 | <0.1 |
| 18 | 6.6 | 1.7 | 7.6 | 3.5 | 3.8 | <0.1 | 0.1 | <0.1 | 9.2 | 1.3 | 1.2 | <0.1 | 2.1 | <0.1 | 8.5 | <0.1 |
| 21 | 5.1 | 2.4 | 3.5 | 4.0 | 8.8 | 5.5 | 9.4 | <0.1 | 4.2 | 2.8 | 5.8 | 10.0 | 5.1 | 4.8 | 5.1 | 7.2 |
| 22 | 17.3 | 14.2 | 16.4 | 6.7 | 22.3 | 6.0 | 17.8 | <0.1 | 21.5 | 11.0 | 20.7 | 6.0 | 14.5 | 5.7 | 17.8 | 9.5 |
| 24 | 2.2 | <0.1 | 2.2 | <0.1 | 0.3 | <0.1 | 3.0 | <0.1 | 1.4 | 0.8 | 0.2 | <0.1 | <0.1 | <0.1 | <0.1 | <0.1 |
*Underlined percentages are families showing an increase of expression of at least 50% in SEA-stimulated samples in comparison with cells cultured in medium only. Results for BV expanded subfamilies and for BV1 are shown.