Activation of Adult or CB Lymphocytes With Bacterial Toxins
| Cells* . | Phenotype . | ||||||
|---|---|---|---|---|---|---|---|
| 3H-Thymidine† . | CD3 . | CD4 . | CD8 . | CD25 . | CD45RA . | CD45RO . | |
| Adult PBL (n = 4) | |||||||
| d0 | 74 ± 1.5‡ | 48 ± 4 | 33 ± 3 | 2.6 ± 2 | 68 ± 9 | 54.6 ± 1.7 | |
| d4 SEA | 63 ± 11.5 | 81 ± 7 | 53 ± 15 | 33 ± 3.2 | 36 ± 11 | 74.5 ± 5 | 53 ± 8.6 |
| d4 TSST-1 | 40 ± 15.1 | 75 ± 2.5 | 44 ± 14 | 31.2 ± 4 | 30 ± 0.4 | 68.6 ± 4 | 43 ± 10 |
| CB leucocytes (n = 4) | |||||||
| d0 | 43 ± 10.7 | 44 ± 10.8 | 21 ± 7.3 | 3 ± 1.5 | 55 ± 7.9 | 7.5 ± 3.2 | |
| d4 SEA | 60 ± 25.1 | 73 ± 2.1 | 49 ± 10 | 29 ± 6.5 | 44 ± 4.5 | 72 ± 9 | 50 ± 3 |
| d4 TSST-1 | 50 ± 16.1 | 69 ± 6 | 53 ± 12 | 22 ± 1.5 | 40 ± 6.4 | 72 ± 18 | 63 ± 8 |
| Cells* . | Phenotype . | ||||||
|---|---|---|---|---|---|---|---|
| 3H-Thymidine† . | CD3 . | CD4 . | CD8 . | CD25 . | CD45RA . | CD45RO . | |
| Adult PBL (n = 4) | |||||||
| d0 | 74 ± 1.5‡ | 48 ± 4 | 33 ± 3 | 2.6 ± 2 | 68 ± 9 | 54.6 ± 1.7 | |
| d4 SEA | 63 ± 11.5 | 81 ± 7 | 53 ± 15 | 33 ± 3.2 | 36 ± 11 | 74.5 ± 5 | 53 ± 8.6 |
| d4 TSST-1 | 40 ± 15.1 | 75 ± 2.5 | 44 ± 14 | 31.2 ± 4 | 30 ± 0.4 | 68.6 ± 4 | 43 ± 10 |
| CB leucocytes (n = 4) | |||||||
| d0 | 43 ± 10.7 | 44 ± 10.8 | 21 ± 7.3 | 3 ± 1.5 | 55 ± 7.9 | 7.5 ± 3.2 | |
| d4 SEA | 60 ± 25.1 | 73 ± 2.1 | 49 ± 10 | 29 ± 6.5 | 44 ± 4.5 | 72 ± 9 | 50 ± 3 |
| d4 TSST-1 | 50 ± 16.1 | 69 ± 6 | 53 ± 12 | 22 ± 1.5 | 40 ± 6.4 | 72 ± 18 | 63 ± 8 |
*Adult or CB leukocytes were either unstimulated (d0) or stimulated with bacterial toxins SEA or TSST-1 (1 ng/mL) during 4 days before 3H-thymidine incorporation measurement or immunophenotype analysis.
3H-thymidine incorporation (cpm × 10−3) measured at d4. 3H thymidine incorporation in unstimulated adult or CB lymphocytes at d4 was <1,000 cpm.
Percent of positive cells of the indicated specificity ± SD.