Long-Term Culture Conditions
| Feeder Layer . | Soluble Cytokines Added . |
|---|---|
| (human cytokines produced, ng/mL) . | (final concentration)* . |
| HMF† | — |
| Sl/Sl‡ | — |
| Sl/Sl JIL-3ρ (IL-3, 16.5 ng/mL) | SF (50 ng/mL) |
| Sl/Sl | FL (50 ng/mL) |
| Sl/Sl JIL-3 (IL-3, 16.5 ng/mL) | FL (50 ng/mL) |
| Mixed2-155 (IL-3, 2.5 ng/mL; G-CSF, 95 ng/mL; SF, 2 ng/mL) | — |
| Mixed2-155 (IL-3, 2.5 ng/mL; G-CSF, 95 ng/mL; SF, 2 ng/mL) | FL (50 ng/mL) |
| Feeder Layer . | Soluble Cytokines Added . |
|---|---|
| (human cytokines produced, ng/mL) . | (final concentration)* . |
| HMF† | — |
| Sl/Sl‡ | — |
| Sl/Sl JIL-3ρ (IL-3, 16.5 ng/mL) | SF (50 ng/mL) |
| Sl/Sl | FL (50 ng/mL) |
| Sl/Sl JIL-3 (IL-3, 16.5 ng/mL) | FL (50 ng/mL) |
| Mixed2-155 (IL-3, 2.5 ng/mL; G-CSF, 95 ng/mL; SF, 2 ng/mL) | — |
| Mixed2-155 (IL-3, 2.5 ng/mL; G-CSF, 95 ng/mL; SF, 2 ng/mL) | FL (50 ng/mL) |
Soluble cytokines were added twice weekly to achieve final concentration in LTC medium as shown.
Feeder cells derived from the adherent layer of LTC of normal human marrow.16
Embryonic fibroblast cell line derived from Sl/Sl mice, which are defective in the production of SF.23
ρ Sl/Sl cells that have been genetically engineered to produce human IL-3.
A 1:1 mixture of Sl/Sl cells that have been engineered to produce human IL-3 and SF, and M210B4 cells27 that have been engineered to produce IL-3 and G-CSF.19