Platelet Adhesion to CRPs Under Static Conditions
| Surface . | Coverage . | +MoAb 176D7 . |
|---|---|---|
| Collagen I, M | 21.7 ± 0.5 (n = 8) | 2.4 ± 0.4 (n = 7) |
| Collagen I, F | 21.3 ± 1.2 (n = 7) | 20.0 ± 0.8 (n = 5) |
| CRP 1 | 20.2 ± 0.8 (n = 8) | 19.2 ± 0.3 (n = 6) |
| CRP 1-XL | 28.7 ± 1.1 (n = 6) | 28.2 ± 0.9 (n = 6) |
| CRP 2 | 19.4 ± 0.9 (n = 8) | 18.3 ± 0.4 (n = 6) |
| CRP 2-XL | 25.3 ± 6.0 (n = 6) | 27.6 ± 6.2 (n = 6) |
| 3% BSA | 0.9 ± 0.1 (n = 7) | — |
| Surface . | Coverage . | +MoAb 176D7 . |
|---|---|---|
| Collagen I, M | 21.7 ± 0.5 (n = 8) | 2.4 ± 0.4 (n = 7) |
| Collagen I, F | 21.3 ± 1.2 (n = 7) | 20.0 ± 0.8 (n = 5) |
| CRP 1 | 20.2 ± 0.8 (n = 8) | 19.2 ± 0.3 (n = 6) |
| CRP 1-XL | 28.7 ± 1.1 (n = 6) | 28.2 ± 0.9 (n = 6) |
| CRP 2 | 19.4 ± 0.9 (n = 8) | 18.3 ± 0.4 (n = 6) |
| CRP 2-XL | 25.3 ± 6.0 (n = 6) | 27.6 ± 6.2 (n = 6) |
| 3% BSA | 0.9 ± 0.1 (n = 7) | — |
Data are expressed as the percentage of surface covered by platelets. All peptides are sprayed at a concentration of 10 μg/cm2 on Thermanox coverslips. Collagen I was human placenta monomeric (M) or fibrillar (F) collagen type I sprayed on glass coverslips at 30 μg/cm2. MoAb 176D7, directed against the α2-subunit of the integrin α2β1, was added at a concentration of 20 μg/mL whole blood. Values are the mean ± SEM, obtained in at least two independent experiments.
Abbreviations: XL, cross-linked; n, number of evaluated coverslips.