Binding Site Number and Affinity of Echistatin and Its Analogs to Resting and ADP-Activated Platelets
| . | Platelets (resting) . | Platelets (ADP) . |
|---|---|---|
| Echistatin | 41,890 ± 3,430 | 46,670 ± 5,430 |
| 106.5 ± 18.2 nmol/L | 90.0 ± 18.6 nmol/L | |
| Echistatin D27W | 45,430 ± 6,330 | 47,280 ± 4,400 |
| 64.1 ± 13.8 nmol/L | 55.6 ± 18.0 nmol/L | |
| Echistatin 1-41 | 17,690 ± 1,600 | 38,970 ± 930 |
| 210.3 ± 31.4 nmol/L | 108.2 ± 27.3 nmol/L |
| . | Platelets (resting) . | Platelets (ADP) . |
|---|---|---|
| Echistatin | 41,890 ± 3,430 | 46,670 ± 5,430 |
| 106.5 ± 18.2 nmol/L | 90.0 ± 18.6 nmol/L | |
| Echistatin D27W | 45,430 ± 6,330 | 47,280 ± 4,400 |
| 64.1 ± 13.8 nmol/L | 55.6 ± 18.0 nmol/L | |
| Echistatin 1-41 | 17,690 ± 1,600 | 38,970 ± 930 |
| 210.3 ± 31.4 nmol/L | 108.2 ± 27.3 nmol/L |
The number of binding sites and binding affinity were estimated by Scatchard analysis of 125I binding data from three experiments. The results correspond to mean and standard deviation. Binding affinity from these experiments are expressed in nanomoles per liter.