Marrow Analysis in Leukemic Bone Biopsies Engrafted in SCID Mice
| . | Human BM Biopsy . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | R19 . | R20 . | R28 . | R29 . | R31 . | R35 . | R36 . | R38 . | R39 . | R41 . | R46 . |
| Engraftment period (wk) | 19 | 15 | 8 | 8 | 17 | 10 | 13 | 15 | 9 | 11 | 14 |
| Cytology | 100% CD19+ lymphoblasts | 68% CD19+ lymphoblasts | 19% CD19+ lymphoblasts | 58% CD19+ | |||||||
| lymphoblasts | 20% myeloblasts | 100% lymphoblasts | Dead cells | 15% myeloblasts | Rare blasts | 91% CD19+ lymphoblasts | 95% myeloblasts | ||||
| Histology | ND | ND | ND | ND | Small foci of blasts showing signs of myeloid differentiation; myelofibrosis | Diffuse blastic infiltration; myelofibrosis | No blasts; myelofibrosis | Rare blasts; moderate myelofibrosis | Rare blasts; moderate myelfibrosis | Partial blastic infiltration (2/3) with adipocytes; myelofibrosis | Diffuse blastic infiltration with signs of myeloid differentiation; myelofibrosis |
| . | Human BM Biopsy . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | R19 . | R20 . | R28 . | R29 . | R31 . | R35 . | R36 . | R38 . | R39 . | R41 . | R46 . |
| Engraftment period (wk) | 19 | 15 | 8 | 8 | 17 | 10 | 13 | 15 | 9 | 11 | 14 |
| Cytology | 100% CD19+ lymphoblasts | 68% CD19+ lymphoblasts | 19% CD19+ lymphoblasts | 58% CD19+ | |||||||
| lymphoblasts | 20% myeloblasts | 100% lymphoblasts | Dead cells | 15% myeloblasts | Rare blasts | 91% CD19+ lymphoblasts | 95% myeloblasts | ||||
| Histology | ND | ND | ND | ND | Small foci of blasts showing signs of myeloid differentiation; myelofibrosis | Diffuse blastic infiltration; myelofibrosis | No blasts; myelofibrosis | Rare blasts; moderate myelofibrosis | Rare blasts; moderate myelfibrosis | Partial blastic infiltration (2/3) with adipocytes; myelofibrosis | Diffuse blastic infiltration with signs of myeloid differentiation; myelofibrosis |
BM implants in SCID mice were analyzed by flow cytometry or on tissue sections and cell smears. The 3 approaches were used simultaneously when permitted by the size of the graft.