Table 2.
PCR Conditions for Different Sets of Primers
Primer
.
Hybridization Temperature (°C)
.
MgCl
2
Concentration (mmol/L)
.
Mean Size of the Products (bp)
.
Vγ1L-J1J2Lc
63
2.5
295
Vγ9L-J1J2Lc
63
2.5
270
Vδ2c-Dδ3-AS
61
2.5
250
VH-JHc
58
2.5
96
Primer
.
Hybridization Temperature (°C)
.
MgCl
2
Concentration (mmol/L)
.
Mean Size of the Products (bp)
.
Vγ1L-J1J2Lc
63
2.5
295
Vγ9L-J1J2Lc
63
2.5
270
Vδ2c-Dδ3-AS
61
2.5
250
VH-JHc
58
2.5
96
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