Table 1. Expansion of Marrow CD34 CD38... | American Society of Hematology

Table 1.

Expansion of Marrow CD34⁺ CD38^dim Cells and CD34⁺ CD38⁺ Cells in Liquid Culture

Cytokines	Cell Yield (fold-increase ± SEM)
	CD34⁺ CD38^dim		CD34⁺ CD38⁺
	Average Fold Increase^*	FL Enhancement^†	Average Fold Increase^*	FL Enhancement^†
IL-3 + IL-6	3.8 ± 2.2		267 ± 141
IL-3 + IL-6 + FL	280 ± 129	50.0 ± 22.6^‡	1,467 ± 873	5.56 ± 2.08
		(P = .15)
IL-3 + IL-6 + KL	1,333 ± 857		3,244 ± 2,320
IL-3 + IL-6 + KL + FL	6,867 ± 2,059ρ	14.6 ± 5.2^1-155	4,243 ± 1,980	1.89 ± 0.54
		(P = .034)

Cytokines	Cell Yield (fold-increase ± SEM)
	CD34⁺ CD38^dim		CD34⁺ CD38⁺
	Average Fold Increase^*	FL Enhancement^†	Average Fold Increase^*	FL Enhancement^†
IL-3 + IL-6	3.8 ± 2.2		267 ± 141
IL-3 + IL-6 + FL	280 ± 129	50.0 ± 22.6^‡	1,467 ± 873	5.56 ± 2.08
		(P = .15)
IL-3 + IL-6 + KL	1,333 ± 857		3,244 ± 2,320
IL-3 + IL-6 + KL + FL	6,867 ± 2,059ρ	14.6 ± 5.2^1-155	4,243 ± 1,980	1.89 ± 0.54
		(P = .034)

Average fold increase in total cells was calculated by averaging absolute peak values from 6 or 7 trials (Fig 2).

†

Fold enhancement of cell growth observed when FL was added to either [IL-3 + IL-6] or [IL-3 + IL-6 + KL]. P values show significance of difference of enhancement of CD34⁺ CD38^dim versus CD34⁺ CD38⁺ fractions.

‡

The modest P value reflects the highly variable response obtained with CD34⁺ CD38^dim cells from different donors when cultured in [IL-3 + IL-6 + FL] (Fig 2). In columns 3 and 5, FL enhancement was calculated by dividing (fold cell increase with FL) by (fold cell increase without FL) in each experiment and averaging the results. This calculation reduces the impact of donor variability. Resultant values for FL stimulation consequently differ somewhat from those obtained by comparing entries in column 2 or in column 4.

ρ For CD34⁺ CD38^dim cells, growth in [IL-3 + IL-6 + KL + FL] was significantly greater than in [IL-3 + IL-6] (P = .012), [IL-3 + IL-6 + FL] (P = .009), and [IL-3 + IL-6 + KL] (P = .047).

F1-155

Addition of FL to [IL-3 + IL-6 + KL] enhanced the growth of CD34⁺ CD38^dim cells significantly more than the growth of CD34⁺ CD38⁺ cells (P = .034).

View Large

This Feature Is Available To Subscribers Only