Day 7 In Vitro Cytokine Production After BMT
| . | Conditioning Regimen . | |
|---|---|---|
| . | 900 cGy TBI . | 1,300 cGy TBI . |
| TNFα* | ||
| Allogeneic | 1,938 ± 151 | 3,110 ± 219† |
| Syngeneic | 749 ± 9 | 1,046 ± 47† |
| IL-1β* | ||
| Allogeneic | 87 ± 5.5 | 154 ± 3.1† |
| Syngeneic | 6 ± 4.1 | 72 ± 5.1† |
| . | Conditioning Regimen . | |
|---|---|---|
| . | 900 cGy TBI . | 1,300 cGy TBI . |
| TNFα* | ||
| Allogeneic | 1,938 ± 151 | 3,110 ± 219† |
| Syngeneic | 749 ± 9 | 1,046 ± 47† |
| IL-1β* | ||
| Allogeneic | 87 ± 5.5 | 154 ± 3.1† |
| Syngeneic | 6 ± 4.1 | 72 ± 5.1† |
Macrophages were pooled from 4 animals in each group and plated at 105 cells per well in the presence of LPS (0.1 μg/mL). Supernatants were taken at 4 (TNFα) and 48 (IL-1β) hours and analyzed by ELISA. Data represent the mean ± standard error of quadruplicate wells standardized for macrophage number.
In vitro TNFα and IL-1β production (in picograms) per 105 macrophages.
P < .05 v 900 cGy TBI.