NK Cell Colony Formation From Individually Plated Cells
| Exp. # . | No. of Cells Plated . | No. of Colonies . | |||
|---|---|---|---|---|---|
| . | . | Mixed-NK . | Pure-NK . | Pre-B . | Myeloid . |
| Exp. 1 | 196 | 27 | 4 | 13 | 43 |
| Exp. 2 | 193 | 24 | 5 | 18 | 36 |
| Exp. # . | No. of Cells Plated . | No. of Colonies . | |||
|---|---|---|---|---|---|
| . | . | Mixed-NK . | Pure-NK . | Pre-B . | Myeloid . |
| Exp. 1 | 196 | 27 | 4 | 13 | 43 |
| Exp. 2 | 193 | 24 | 5 | 18 | 36 |
Sca-1+c-kit+ cells purified from fetal liver were individually plated into methylcellulose media by micromanipulation. Cells were cultured in the presence of IL-2, IL-7, IL-11, and SF. Colonies were scored after 18 days of incubation.