Peripheral Blood CD4+ and CD8+ T Cells Are Donor (Male)-Derived as Determined by PCR
| Animal . | CD4+ . | CD8+ . |
|---|---|---|
| A89163 (female with male donor) | ||
| cells per PCR reaction | ||
| 1,000 | 6/6 | 5/5 |
| 100 | 12/12 | 12/12 |
| 10 | 12/12 | 11/11 |
| A91382 (female with male donor) | ||
| cells per PCR reaction | ||
| 1,000 | 6/6 | 6/6 |
| 100 | 6/6 | 6/6 |
| 10 | 6/6 | 5/5 |
| Animal . | CD4+ . | CD8+ . |
|---|---|---|
| A89163 (female with male donor) | ||
| cells per PCR reaction | ||
| 1,000 | 6/6 | 5/5 |
| 100 | 12/12 | 12/12 |
| 10 | 12/12 | 11/11 |
| A91382 (female with male donor) | ||
| cells per PCR reaction | ||
| 1,000 | 6/6 | 6/6 |
| 100 | 6/6 | 6/6 |
| 10 | 6/6 | 5/5 |
Values are the number of positive PCRs/the number of samples tested. The cells were isolated from blood of A89163 5.3 years after transplantation and from A91382 4.2 years after transplantation. Cells were purified by two cycles of FACS. The purified cell populations were ≥99% of the desired phenotype. Samples were assayed by PCR for the 174-bp male-specific sequence.