Analysis of CML Patient Samples Using Triple-Probe/Three-Color FISH System
| Patient No. . | Initials . | Age*/Sex . | Ph+/Total . | BM FISH Results . | PBG FISH Results . | ||
|---|---|---|---|---|---|---|---|
| . | . | . | Metaphases† . | Interphase . | BCR-ABLρ . | Interphase . | BCR-ABLρ . |
| . | . | . | . | Cells Scored‡ . | . | Cells Score‡ . | . |
| 1 | K.S. | 42/M | 0/17 | 200 | 0 | 200 | 0 |
| 2 | D.W. | 58/M | 0/20 | 200 | 4 (2%) | 200 | 8 (4%) |
| 3 | S.L. | 46/F | 4/10 (40%) | 100 | 39 (39%) | 42 | 16 (38%) |
| 4 | S.L. | 46/F | 10/10 (100%) | 100 | 99 (99%) | 100 | 64 (64%) |
| 5 | R.C. | 47/M | 9/10 (90%) | 100 | 93 (93%) | 100 | 98 (98%) |
| 6 | G.D. | 69/M | 10/10 (100%) | 100 | 96 (96%) | 100 | 67 (67%) |
| Patient No. . | Initials . | Age*/Sex . | Ph+/Total . | BM FISH Results . | PBG FISH Results . | ||
|---|---|---|---|---|---|---|---|
| . | . | . | Metaphases† . | Interphase . | BCR-ABLρ . | Interphase . | BCR-ABLρ . |
| . | . | . | . | Cells Scored‡ . | . | Cells Score‡ . | . |
| 1 | K.S. | 42/M | 0/17 | 200 | 0 | 200 | 0 |
| 2 | D.W. | 58/M | 0/20 | 200 | 4 (2%) | 200 | 8 (4%) |
| 3 | S.L. | 46/F | 4/10 (40%) | 100 | 39 (39%) | 42 | 16 (38%) |
| 4 | S.L. | 46/F | 10/10 (100%) | 100 | 99 (99%) | 100 | 64 (64%) |
| 5 | R.C. | 47/M | 9/10 (90%) | 100 | 93 (93%) | 100 | 98 (98%) |
| 6 | G.D. | 69/M | 10/10 (100%) | 100 | 96 (96%) | 100 | 67 (67%) |
Abbreviations: BM, bone marrow; PBF, peripheral blood granulocyte.
Age at time of analysis.
As assessed by G-banding.
For this analysis, only cells that were normal (Fig 4a) or BCR-ABL† (Fig 4b) were scored. All other patterns (Figs 4c, d, and e) were excluded from the analysis.
ρ As defined by the presence of a green-red (BCR-ABL) colocalization and separation of one red-blue (ASS-ABL) doublet. See text for details.