Gelation Concentrations of Mixture of Recombinant HbS Mutants With Mouse Hemolysate
| Hb . | HbS Mutants/Mouse Hemolysate . | |
|---|---|---|
| . | 1/1 . | 1/2 . |
| Natural HbS | 79.1 | 95.0 |
| E6V( β)/E121R( β) | 48.2 | 72.6 |
| D75Y(α)/E6V( β)/E121R( β) | 28.0 | 39.6 |
| D6A(α)/D75Y(α)/E6V( β)/E121R( β) | 12.4 | — |
| Hb . | HbS Mutants/Mouse Hemolysate . | |
|---|---|---|
| . | 1/1 . | 1/2 . |
| Natural HbS | 79.1 | 95.0 |
| E6V( β)/E121R( β) | 48.2 | 72.6 |
| D75Y(α)/E6V( β)/E121R( β) | 28.0 | 39.6 |
| D6A(α)/D75Y(α)/E6V( β)/E121R( β) | 12.4 | — |
All measurements were performed in the presence of dextran in 50 mmol/L potassium phosphate buffer, pH 7.5, at 37°C, as described in the text.