Gelation Concentrations of Natural HbS and Recombinant HbS Mutants
| Hb . | Csat (mg/mL) . |
|---|---|
| Natural HbS | 34.4, 34.9, 36.5 (35.3) |
| E6V( β)/E121R( β) | 24.0, 24.6 (24.3) |
| D75Y(α)/E6V( β)/E121R( β) | 11.4, 12.2 (11.8) |
| D6A(α)/D75Y(α)/E6V( β)/E121R( β) | 7.6, 6.8, 6.6 (7.0) |
| Purified Hb SAD | 6.2, 6.1, 6.1 (6.1) |
| Hb . | Csat (mg/mL) . |
|---|---|
| Natural HbS | 34.4, 34.9, 36.5 (35.3) |
| E6V( β)/E121R( β) | 24.0, 24.6 (24.3) |
| D75Y(α)/E6V( β)/E121R( β) | 11.4, 12.2 (11.8) |
| D6A(α)/D75Y(α)/E6V( β)/E121R( β) | 7.6, 6.8, 6.6 (7.0) |
| Purified Hb SAD | 6.2, 6.1, 6.1 (6.1) |
All measurements were performed in the presence of dextran in 50 mmol/L potassium phosphate buffer, pH 7.5, at 37°C, as described in the text. The average values are in parentheses.