Mass Spectrometric Analysis of Recombinant Hbs
| Hb . | Theory . | Measured . | ||
|---|---|---|---|---|
| . | α-Chain . | β-Chain . | α-Chain . | β-Chain . |
| E6V( β)/E121R( β) | 15,126.4 | 15,864.2 | 15,130 | 15,868 |
| D75Y(α)/E6V( β)/E121R( β) | 15,174.0 | 15,864.2 | 15,178 | 15,868 |
| D6A(α)/D75Y(α)/E6V( β)/E121R( β) | 15,130.4 | 15,864.2 | 15,133 | 15,867 |
| Hb . | Theory . | Measured . | ||
|---|---|---|---|---|
| . | α-Chain . | β-Chain . | α-Chain . | β-Chain . |
| E6V( β)/E121R( β) | 15,126.4 | 15,864.2 | 15,130 | 15,868 |
| D75Y(α)/E6V( β)/E121R( β) | 15,174.0 | 15,864.2 | 15,178 | 15,868 |
| D6A(α)/D75Y(α)/E6V( β)/E121R( β) | 15,130.4 | 15,864.2 | 15,133 | 15,867 |
There is an error of 3 or 4 mass units in the measured mass of both α- and β-chains. It is a systematic error, so we can safely conclude that mutations were successful and that the Hbs were expressed properly.