HLA-A2–Bound Peptides From CD34+ Blast Cells of a Patient With CML
| Precursor Protein . | Sequence* . | Yield† (pmol) . | Location‡ . | Predominant Distribution . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | . | . | . |
| Breakpoint Cluster Region Protein | F | L | L | D | H | L | K | R | V | 43 | C | Widespread | ||
| Ribosomal S6 kinase | Y | M | A | P | E | I | L | M | R | S | 3 | C | Widespread | |
| Interferon α/β receptor α chain | X | I | A | L | F | A | L | P | F | 18 | M | Widespread | ||
| Cathepsin G | F | L | L | P | T | G | A | E | A | 41 | L | Myeloid | ||
| c-pim | L | L | Y | D | M | V | X | G | D | I | P | 23 | C | Myeloid |
| c-fes | I | P | R | A | E | V | A | E | L | L | 9 | C | Myeloid (endothelial) | |
| GM CSF receptor α chain | F | I | Y | N | A | D | L | M | N | X | 21 | M | Myeloid | |
| Globin α chain | V | L | S | P | A | D | K | T | N | V | K | 23 | C | Erythroid |
| Globin β chain | K | V | N | V | D | E | V | G | G | E | 6 | C | Erythroid | |
| Dematin | S | L | P | H | F | H | H | P | E | T | 14 | M | Erythroid | |
| IL-7 | K | Q | Y | E | S | V | L | M | V | S | I | 67 | C | Stromal cell (lymphoid) |
| CD3 ζ chain | L | L | D | P | K | L | X | Y | L | L | 52 | C | T-lymphoid cell | |
| Precursor Protein . | Sequence* . | Yield† (pmol) . | Location‡ . | Predominant Distribution . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | . | . | . |
| Breakpoint Cluster Region Protein | F | L | L | D | H | L | K | R | V | 43 | C | Widespread | ||
| Ribosomal S6 kinase | Y | M | A | P | E | I | L | M | R | S | 3 | C | Widespread | |
| Interferon α/β receptor α chain | X | I | A | L | F | A | L | P | F | 18 | M | Widespread | ||
| Cathepsin G | F | L | L | P | T | G | A | E | A | 41 | L | Myeloid | ||
| c-pim | L | L | Y | D | M | V | X | G | D | I | P | 23 | C | Myeloid |
| c-fes | I | P | R | A | E | V | A | E | L | L | 9 | C | Myeloid (endothelial) | |
| GM CSF receptor α chain | F | I | Y | N | A | D | L | M | N | X | 21 | M | Myeloid | |
| Globin α chain | V | L | S | P | A | D | K | T | N | V | K | 23 | C | Erythroid |
| Globin β chain | K | V | N | V | D | E | V | G | G | E | 6 | C | Erythroid | |
| Dematin | S | L | P | H | F | H | H | P | E | T | 14 | M | Erythroid | |
| IL-7 | K | Q | Y | E | S | V | L | M | V | S | I | 67 | C | Stromal cell (lymphoid) |
| CD3 ζ chain | L | L | D | P | K | L | X | Y | L | L | 52 | C | T-lymphoid cell | |
Standard IUPAC amino acid abbreviations are used. X denotes an unidentifiable PTH-amino acid.
Initial yield of peptides calculated from the repetitive yields of PTH amino acid derivatives of the sequence. The total yield of peptides in Table 1 accounted for approximately 9% of the mass of material found in all the HLA-A2 fractions combined.
C, cytoplasmic; L, lumenal; M, membrane associated.