Binding Constants Calculated From hTFAA Inhibition of FX Activation Catalyzed by Relipidated TF(1-243)⋅FVlla
| (TF[1-243]) . | KA . | Ki . | [I]50 . |
|---|---|---|---|
| (nmol/L) . | (pmol/L) . | (nmol/L) . | (μmol/L) . |
| 0.05 | 8.9 | 10.0 | 0.27 |
| 0.2 | 2.2 | 5.8 | 2.5 |
| 1.0 | 2.5 | 6.5 | 12.1 |
| 5.0 | 2.7 | 5.6 | 48.3 |
| (TF[1-243]) . | KA . | Ki . | [I]50 . |
|---|---|---|---|
| (nmol/L) . | (pmol/L) . | (nmol/L) . | (μmol/L) . |
| 0.05 | 8.9 | 10.0 | 0.27 |
| 0.2 | 2.2 | 5.8 | 2.5 |
| 1.0 | 2.5 | 6.5 | 12.1 |
| 5.0 | 2.7 | 5.6 | 48.3 |
Binding constants (KA , Ki) were calculated by nonlinear regression analysis of the data of Fig 3B by using equation 2. For this analysis KS was set equal to the Km (11.4 μmol/L) determined for human FVlla in the presence of calcium alone,4 and α (0.0048) was calculated from the ratio of the Km values in the presence and absence of relipidated TF.4