Effect of Pre-Tp Plasma and Neutralization of Cytokines on MK Colony Growth
| Plasma Added . | No. of MK Colonies per Culture . |
|---|---|
| Normal mare plasma | 26.7 ± 1.9* |
| Preinfection | 27.5 ± 2.5* |
| Preinfection + anti–TGF-β | 26.8 ± 1.8* |
| Preinfection + anti–TNF-α | 27.9 ± 2.5* |
| Preinfection + anti–TGF-β and anti–TNF-α | 25.9 ± 3.0* |
| Pre-Tp | 8.0 ± 2.6† |
| Pre-Tp + anti–TGF-β | 14.6 ± 2.7‡ |
| Pre-Tp + anti–TNF-α | 12.8 ± 2.9 |
| Pre-Tp + anti–TGF-β and anti–TNF-α | 15.2 ± 0.8‡ |
| Pre-Tp + antibody control | 8.3 ± 2.9† |
| Plasma Added . | No. of MK Colonies per Culture . |
|---|---|
| Normal mare plasma | 26.7 ± 1.9* |
| Preinfection | 27.5 ± 2.5* |
| Preinfection + anti–TGF-β | 26.8 ± 1.8* |
| Preinfection + anti–TNF-α | 27.9 ± 2.5* |
| Preinfection + anti–TGF-β and anti–TNF-α | 25.9 ± 3.0* |
| Pre-Tp | 8.0 ± 2.6† |
| Pre-Tp + anti–TGF-β | 14.6 ± 2.7‡ |
| Pre-Tp + anti–TNF-α | 12.8 ± 2.9 |
| Pre-Tp + anti–TGF-β and anti–TNF-α | 15.2 ± 0.8‡ |
| Pre-Tp + antibody control | 8.3 ± 2.9† |
Equine marrow cells were cultured in triplicate in agar clots and MK colonies were counted on day 7. Mean MK colony number is expressed per 1 × 105 cells plated. The data shown are the mean values for 16 foals. SCID and immunocompetent foal values were not statistically different; thus, data were pooled. Colony numbers were not significantly different using the two antibody controls (murine isotype-matched monoclonal and rabbit polyclonal antiserum) separately or in combination, so the antibody control category is a pooled value. Differences between means were statistically significant (P < .05), except where noted.
No significant difference between these means.
No significant difference between these means.
No significant difference between these means.