List of Cell Lines and Patient Samples, Abbreviated Karyotype, Results of Southern Blot With JH Probe, and Corresponding LDI-PCR Products for Given Enzyme Digest
| . | Diagnosis . | Karyotype . | Digest . | Size of IGHJ Fragments . | Size of LDI-PCR Products . | ||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | . |
| BCL2 | |||||||||
| Case no. 1 | B-CLL | t(14; 18)(q32;q21) | H | 2.5 | 3.9 | 9.9(g) | 0.7* | 2.1 | |
| DoHH2 | B-NHL | t(8; 14; 18)(q24;q32; q21) | H | 4.7 | 4.8 | 2.9 | 3.0* | ||
| Granta 452 | Transformed B-NHL | t(9; 14; 18)(p13; q32; q21) | P | 4.7 | 4.9 | 2.5* | 2.7 | ||
| Karpas 231 | B-ALL | t(14; 18)(q32; q21) | Bg | 3.9 | 4.6 | 2.9* | 3.6 | ||
| Karpas 353 | B-ALL | t(14; 18)(q32; q21) | H | 3.6 | 4.7 | 1.8 | 2.9* | ||
| SU-DUL5 | Lymphoblastic lymphoma | t(8; 18; 14)(q24; q21; q32) | X | 3.7 | 4.9 | 1.5* | 2.7 | ||
| BCL1 | |||||||||
| Case no. 2 | Mc-NHL | t(11; 14)(q13; q32) | H | 3.3 | 4.8 | 9.9(G) | 1.5* | 3.0 | |
| Case no. 3 | B-NHL | ?t(11; 14) | P | 2.8 | 4.4(g) | 5.0 | 0.6 | 2.2(g) | 2.8* |
| Granta 519 | Transformed Mc-NHL | t(11; 14)(q13; q32.3) | Bg | 3.2 | 6.4 | 2.2 | 5.4* | ||
| NCEB-1 | Mc-NHL | t(11; 14)(q13; q32) | Bg | 3.7(G) | 3.9 | 2.7(G) | 2.9 | ||
| BCL7A | |||||||||
| Wien 133 | Burkitt's | t(8; 14; 12)(q24; q32; q24) | S | 2.3(U) | 5.6 | 5.9 | — | 3.8* | 4.1 |
| Others | |||||||||
| Case no. 4 | B-NHL | t(1; 14)(p22; q32), ?t(9; 14)(p13; q32) | P | 4.2 | 4.4 | 2.0 | 2.2 | ||
| Case no. 5 | B-CLL | t(10; 14)(q22; q32), t(14; 19)(q32; q13) | Bg | 3.4 | 3.7(g) | 2.4 | 2.7(g) | ||
| . | Diagnosis . | Karyotype . | Digest . | Size of IGHJ Fragments . | Size of LDI-PCR Products . | ||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | . |
| BCL2 | |||||||||
| Case no. 1 | B-CLL | t(14; 18)(q32;q21) | H | 2.5 | 3.9 | 9.9(g) | 0.7* | 2.1 | |
| DoHH2 | B-NHL | t(8; 14; 18)(q24;q32; q21) | H | 4.7 | 4.8 | 2.9 | 3.0* | ||
| Granta 452 | Transformed B-NHL | t(9; 14; 18)(p13; q32; q21) | P | 4.7 | 4.9 | 2.5* | 2.7 | ||
| Karpas 231 | B-ALL | t(14; 18)(q32; q21) | Bg | 3.9 | 4.6 | 2.9* | 3.6 | ||
| Karpas 353 | B-ALL | t(14; 18)(q32; q21) | H | 3.6 | 4.7 | 1.8 | 2.9* | ||
| SU-DUL5 | Lymphoblastic lymphoma | t(8; 18; 14)(q24; q21; q32) | X | 3.7 | 4.9 | 1.5* | 2.7 | ||
| BCL1 | |||||||||
| Case no. 2 | Mc-NHL | t(11; 14)(q13; q32) | H | 3.3 | 4.8 | 9.9(G) | 1.5* | 3.0 | |
| Case no. 3 | B-NHL | ?t(11; 14) | P | 2.8 | 4.4(g) | 5.0 | 0.6 | 2.2(g) | 2.8* |
| Granta 519 | Transformed Mc-NHL | t(11; 14)(q13; q32.3) | Bg | 3.2 | 6.4 | 2.2 | 5.4* | ||
| NCEB-1 | Mc-NHL | t(11; 14)(q13; q32) | Bg | 3.7(G) | 3.9 | 2.7(G) | 2.9 | ||
| BCL7A | |||||||||
| Wien 133 | Burkitt's | t(8; 14; 12)(q24; q32; q24) | S | 2.3(U) | 5.6 | 5.9 | — | 3.8* | 4.1 |
| Others | |||||||||
| Case no. 4 | B-NHL | t(1; 14)(p22; q32), ?t(9; 14)(p13; q32) | P | 4.2 | 4.4 | 2.0 | 2.2 | ||
| Case no. 5 | B-CLL | t(10; 14)(q22; q32), t(14; 19)(q32; q13) | Bg | 3.4 | 3.7(g) | 2.4 | 2.7(g) | ||
Abbreviations: G, germline band; g, weak germline band observed in clinical cases due to contaminating normal cells; U, unamplified band; Bg, Bgl II; H, HindIII; P, Pst I; S, Sac I; X, Xba I; Mc-NHL, mantle-cell lymphoma.