Effect of rD-mPGPtide on Reconstitution of Donor CD4+ and CD8+T Cells in the Haploidentical and MHC Class II-Disparate Strain Combinations
| Strain Combination . | Treatment . | Phenotype of Spleen (% donor/host) . | Phenotype of LN (% donor/host) . | ||
|---|---|---|---|---|---|
| . | . | CD4+ Cells . | CD8+ Cells . | CD4+ Cells . | CD8+ Cells . |
| (B6D2)F1 → (B6CB)F1 (800 cGy) | PBS | 4/22 | 3/10 | 4/35 | 2/18 |
| rD-mPGPtide | 28/10 | 8/2 | 44/16 | 20/2 | |
| Anti-CD4 MoAb | 24/9 | 7/2 | 44/16 | 18/2 | |
| bm12 → B6-Ly5.2 (750 cGy) | PBS | 6/28 | 2/21 | 6/45 | 3/23 |
| rD-mPGPtide | 17/11 | 7/6 | 32/15 | 13/10 | |
| Anti-CD4 MoAb | 15/10 | 5/4 | 28/14 | 10/7 | |
| Strain Combination . | Treatment . | Phenotype of Spleen (% donor/host) . | Phenotype of LN (% donor/host) . | ||
|---|---|---|---|---|---|
| . | . | CD4+ Cells . | CD8+ Cells . | CD4+ Cells . | CD8+ Cells . |
| (B6D2)F1 → (B6CB)F1 (800 cGy) | PBS | 4/22 | 3/10 | 4/35 | 2/18 |
| rD-mPGPtide | 28/10 | 8/2 | 44/16 | 20/2 | |
| Anti-CD4 MoAb | 24/9 | 7/2 | 44/16 | 18/2 | |
| bm12 → B6-Ly5.2 (750 cGy) | PBS | 6/28 | 2/21 | 6/45 | 3/23 |
| rD-mPGPtide | 17/11 | 7/6 | 32/15 | 13/10 | |
| Anti-CD4 MoAb | 15/10 | 5/4 | 28/14 | 10/7 | |
Data from one experiment representative of three separate experiments are presented as the percentage of positive cells, as analyzed by flow cytometry. Chimerism was determined using the following MoAb pairs: PE-anti-H2Kd, FITC-anti-CD4 (or CD8) and FITC-anti-H2Kk, PE-anti-CD4 (or CD8) [(B6D2)F1 → (B6CB)F1 ]; FITC-anti-CD45.1 (anti-Ly5.1) and PE-anti-CD4 (or CD8) (bm → B6-Ly5.2). Mice in the (B6D2)F1 → (B6CB)F1 and the bm12 → B6-Ly5.2 strain combinations were analyzed on days 72 and 60 after transplantation, respectively. The mean of samples from five mice per group is presented for each individual treatment. The values in all rD-mPGPtide and anti-CD4 MoAb treatment groups were significantly different from those in the PBS control group, P ≤ .01.