HCMV DNA Presence, Gene Expression, and Virus Production in BM Cell Progeny Derived From CD34+ Cells Infected With Clinical Isolate
| Subject . | Days After Infection . | |||
|---|---|---|---|---|
| . | 7 . | 14 . | 21 . | 28 . |
| Viral DNA | + | + | + | + |
| IE mRNA | + | + | + | + |
| L mRNA | − | − | + | + |
| Viral proteins | − | − | − | + |
| Infectious virus | − | − | − | + |
| Subject . | Days After Infection . | |||
|---|---|---|---|---|
| . | 7 . | 14 . | 21 . | 28 . |
| Viral DNA | + | + | + | + |
| IE mRNA | + | + | + | + |
| L mRNA | − | − | + | + |
| Viral proteins | − | − | − | + |
| Infectious virus | − | − | − | + |
Virus titer was determined using routine plaque assay with HEL cells. Supernatants from HCMV-infected and mock-infected CD34+-derived macrophages were cocultured with HEL cells for 14 days. Virus titer recovered from the supernatants of the macrophages derived from CD34+ cells infected with clinical isolate was 3 × 10 PFU/mL. No virus was recovered from the supernatants of the macrophages derived from CD34+ cells infected with recombinant laboratory strain.