Frequency of HCMV IE and L Antigens in HCMV-Infected CD34+-Derived Macrophages
| Virus . | % of Cells Positive* for Viral IE and L Antigens at 28 d After Infection . | |
|---|---|---|
| . | IE . | L . |
| Clinical isolate | 50 | 30 |
| Towne/lox2 | 30 | 20 |
| Virus . | % of Cells Positive* for Viral IE and L Antigens at 28 d After Infection . | |
|---|---|---|
| . | IE . | L . |
| Clinical isolate | 50 | 30 |
| Towne/lox2 | 30 | 20 |
A minimum of 200 cells were counted. IE and L HCMV antigens were detected by immunostaining using alkaline-phosphatase substrate KIT (Vector Laboratories Inc).