Table 2.

Inhibitory Effect of HCMV on the Colony Formation by CD34+ Progenitor Cells

Virus% Inhibition of Colony Formation
CFU-MixBFU-ECFU-GM
Clinical isolate 52 ± 17 23 ± 14 58 ± 11 
Towne/lox2  22 ± 12 10 ± 6 26 ± 15 
Virus% Inhibition of Colony Formation
CFU-MixBFU-ECFU-GM
Clinical isolate 52 ± 17 23 ± 14 58 ± 11 
Towne/lox2  22 ± 12 10 ± 6 26 ± 15 

Values are presented as mean percentage ± SEM of four experiments performed. The actual number of colonies detected in mock-infected control cultures ranged from 84 to 116 for myeloid colonies (CFU-GM), from 10 to 58 for erythroid colonies (BFU-E), and from 104 to 174 for the total amount of colonies (CFU-Mix) per well of 6-well plate. Highly purified CD34+ BM cells were plated immediately after infection at a density of 7 × 103 cells per well of a 6-well plate in methylcellulose. Cells were either mock-infected or infected at a multiplicity of infection of 100 PFU/cell with clinical isolate 95(2) or laboratory strain Towne/lox2. The cultures were stimulated with 50 ng/mL IL-3, 50 ng/mL GM-CSF, 50 ng/mL M-CSF, 50 ng/mL SCF, and 10 U/mL Epo. Colonies were counted after 14 days in culture.

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