Primers and Predicted Product Sizes for PCR Analysis
| Gene . | Primer Sequence* . | Size of PCR Product (bp) . | Reference . | |
|---|---|---|---|---|
| . | . | DNA . | cDNA . | . |
| IE | I 5′-GAGTCCTCTGCCAAGAGAAA-3′ | 332 | 218 | Sodeberg and Larson44 |
| I 5′-GAGTTCTGCCAGGACATCTTT-3′ | ||||
| II 5′-GAGAAAGATGGACCCTGATAAT-3′ | ||||
| II 5′-CTCGGGTTCTCGTTGCAAT-3′ | ||||
| MCP | 5′-TCCGCACTTTCGTAGCAC-3′ | 638 | 638 | |
| 5′-ACCGCTTGTGCTCTCTCTTT-3′ | ||||
| β-actin | 5′-GTGGGGCGCCCCAGGCACCA-3′ | 548 | 548 | Brenner et al48 |
| 5′-CTCCTTAATGTCACGCACGATTTC-3′ | ||||
| β-gal | 5′-CCGACGTGACGCGAGTATTAC-3′ | 900 | 900 | |
| 5′-GCTGCAAGGCGATTAAGT-3′ | ||||
| Gene . | Primer Sequence* . | Size of PCR Product (bp) . | Reference . | |
|---|---|---|---|---|
| . | . | DNA . | cDNA . | . |
| IE | I 5′-GAGTCCTCTGCCAAGAGAAA-3′ | 332 | 218 | Sodeberg and Larson44 |
| I 5′-GAGTTCTGCCAGGACATCTTT-3′ | ||||
| II 5′-GAGAAAGATGGACCCTGATAAT-3′ | ||||
| II 5′-CTCGGGTTCTCGTTGCAAT-3′ | ||||
| MCP | 5′-TCCGCACTTTCGTAGCAC-3′ | 638 | 638 | |
| 5′-ACCGCTTGTGCTCTCTCTTT-3′ | ||||
| β-actin | 5′-GTGGGGCGCCCCAGGCACCA-3′ | 548 | 548 | Brenner et al48 |
| 5′-CTCCTTAATGTCACGCACGATTTC-3′ | ||||
| β-gal | 5′-CCGACGTGACGCGAGTATTAC-3′ | 900 | 900 | |
| 5′-GCTGCAAGGCGATTAAGT-3′ | ||||
Primers I and II are outer and inner primer pairs, respectively. Primers were prepared on an Applied Biosystems DNA synthesizer.