Limiting Numbers of Mature αβ T Cells Are Required for Complete Allogeneic Engraftment in B6 → AKR Chimeras
| Group . | No. of Mice . | Donor αβ T Cells Added . | Donor γδ T Cells Added . | (%) Donor T Cells8-150 . | (Mean) . | (%) Donor (H-2b) Cells8-151 . | (Mean) . |
|---|---|---|---|---|---|---|---|
| I | 3 | None | None | 6, 0, 8 | (5) | 8, 3, 15 | (9) |
| II | 3 | 2.5 × 104 | None | 1, 1, 61 | (21) | 2, 3, 91 | (32) |
| III | 3 | None | 160 × 106 | 59, 59, 64 | (61) | 90, 84, 91 | (88) |
| IV | 3 | 2.5 × 104 | 160 × 106 | 97, 96, 97 | (97) | 98, 99, 99 | (99) |
| Group . | No. of Mice . | Donor αβ T Cells Added . | Donor γδ T Cells Added . | (%) Donor T Cells8-150 . | (Mean) . | (%) Donor (H-2b) Cells8-151 . | (Mean) . |
|---|---|---|---|---|---|---|---|
| I | 3 | None | None | 6, 0, 8 | (5) | 8, 3, 15 | (9) |
| II | 3 | 2.5 × 104 | None | 1, 1, 61 | (21) | 2, 3, 91 | (32) |
| III | 3 | None | 160 × 106 | 59, 59, 64 | (61) | 90, 84, 91 | (88) |
| IV | 3 | 2.5 × 104 | 160 × 106 | 97, 96, 97 | (97) | 98, 99, 99 | (99) |
Irradiated (800 cGy) AKR recipients were transplanted with 10 × 106 TCD B6 BM with or without the indicated number of activated γδ T cells from β0 donors (pure B6 background) or naive αβ T cells from B6 donors. After ex vivo activation, 99.4% of cultured cells expressed the γδ TCR. Numbers represent data for individual mice. Donor T cells were defined as Thy 1.2+ CD3+ and donor cells as H-2b+. Animals were evaluated 35 to 37 days posttransplant.
Key statistical comparisons: group I v II, P = .50, groups III and IV v I, P < .001; group III v IV; P < .0001.
Group I v II, P = .51, groups III and IV v I, P < .002; group III v IV, P = .043.