NPM-ALK Fish Assay of Lymphoma Biopsy Samples
| Accession No. . | Date of Specimen . | Tissue . | Diagnosis . | FISH Results* . | Karyotype . |
|---|---|---|---|---|---|
| t(2; 5) Positive Cases | |||||
| 931762 | 02/11/93 | LN | ALCL (Indeterminant) | + | 46,XX,t(2; 5)(p23;q35)[3]/92,XXXX,t(2; 5)(p23;q35)x2[17] |
| 100/150 = 66% | |||||
| 910490 | 09/07/90 | LN | ALCL (T cell) | + | 91,XXXX,+i(1)(q10),t(2; 5)(p23;q35)x2,−6,−9,−10, −11,+12,−13,del(17)(p11),add(19)(q13),+add(20) (p13),+del(20)(q12),−21,−22,+mar1,+mar2[17]/46,XX[3] |
| 932394 | 04/28/93 | LN | ALCL (T cell) | + | 91,XXYY,i(1q),−2,t(2; 5)(p23;q35)x2,−4,+5,+6,−7x2, −11,add(15)(p11),−17,−21,+mar1,+mar2, +mar3x2[19]/46,XY[2] |
| 930926 | 10/29/92 | LN | Immunoblastic lymphoma (T cell) | + | 47,XY,+X,t(2; 5)(p23;q35)[4]/46,XY[3] |
| 47/100 = 47% | |||||
| 911489 | 01/21/91 | LN | Diffuse LCL (T cell) | + | 46,XY,t(2; 5)(p23;q35)[9]/45,X,−Y,t(2; 5)(p23;q35)[5]/46,XY[6] |
| 931737 | 02/09/93 | BM | Diffuse LCL (T cell) | + | 47,XY,t(2; 5)(p23;q35),+7[2]/46,XY[18] |
| 24/150 = 16% | |||||
| 922873 | 06/18/92 | LN | Diffuse LCL (T cell) | + | 47,XY,t(2; 5)(p23;q35),+7[20] |
| 932499 | 05/13/93 | LN | Follicular mixed cell lymphoma (B cell) | + | 50,XX,t(2; 5)(p23;q35),−3,+del(3)(p25)x3,del(13) (q22),+18,+22[cp5]/46,XX[12] Control Cases |
| 36/150 = 24% | |||||
| 870797 | 12/10/86 | LN | HD | − | 46,XY[20] |
| 14/150 = 9% | |||||
| 911100 | 11/20/90 | LN | HD | − | 46,XX[1] |
| 922401 | 04/23/92 | LN | HD | − | 46,XY[20] |
| 9/100 = 9% | |||||
| 932553 | 05/17/93 | LN | HD | − | 46,XY[20] |
| 871261 | 03/11/87 | LN | Follicular mixed cell lymphoma (B cell) | − | 46,XX,t(14; 18)(q32;q21)[2]/46,XX[1] |
| 930808 | 10/14/92 | LN | Follicular mixed cell lymphoma (B cell) | − | 46,XX,inv(9)(p24q32),t(14; 18)(q32;q21)[4]/46,XX[10] |
| 7/100 = 7% | |||||
| 921332† | 12/10/91 | LN | Diffuse LCL (B cell) | − | 87,XX,−Yx2,−1,dup(1)(q21q25),del(2)(p23),+del(2) (p23),−4,−6,+7x2,−9,−11,−17[10]/46,XY[3] |
| 4/100 = 4% |
| Accession No. . | Date of Specimen . | Tissue . | Diagnosis . | FISH Results* . | Karyotype . |
|---|---|---|---|---|---|
| t(2; 5) Positive Cases | |||||
| 931762 | 02/11/93 | LN | ALCL (Indeterminant) | + | 46,XX,t(2; 5)(p23;q35)[3]/92,XXXX,t(2; 5)(p23;q35)x2[17] |
| 100/150 = 66% | |||||
| 910490 | 09/07/90 | LN | ALCL (T cell) | + | 91,XXXX,+i(1)(q10),t(2; 5)(p23;q35)x2,−6,−9,−10, −11,+12,−13,del(17)(p11),add(19)(q13),+add(20) (p13),+del(20)(q12),−21,−22,+mar1,+mar2[17]/46,XX[3] |
| 932394 | 04/28/93 | LN | ALCL (T cell) | + | 91,XXYY,i(1q),−2,t(2; 5)(p23;q35)x2,−4,+5,+6,−7x2, −11,add(15)(p11),−17,−21,+mar1,+mar2, +mar3x2[19]/46,XY[2] |
| 930926 | 10/29/92 | LN | Immunoblastic lymphoma (T cell) | + | 47,XY,+X,t(2; 5)(p23;q35)[4]/46,XY[3] |
| 47/100 = 47% | |||||
| 911489 | 01/21/91 | LN | Diffuse LCL (T cell) | + | 46,XY,t(2; 5)(p23;q35)[9]/45,X,−Y,t(2; 5)(p23;q35)[5]/46,XY[6] |
| 931737 | 02/09/93 | BM | Diffuse LCL (T cell) | + | 47,XY,t(2; 5)(p23;q35),+7[2]/46,XY[18] |
| 24/150 = 16% | |||||
| 922873 | 06/18/92 | LN | Diffuse LCL (T cell) | + | 47,XY,t(2; 5)(p23;q35),+7[20] |
| 932499 | 05/13/93 | LN | Follicular mixed cell lymphoma (B cell) | + | 50,XX,t(2; 5)(p23;q35),−3,+del(3)(p25)x3,del(13) (q22),+18,+22[cp5]/46,XX[12] Control Cases |
| 36/150 = 24% | |||||
| 870797 | 12/10/86 | LN | HD | − | 46,XY[20] |
| 14/150 = 9% | |||||
| 911100 | 11/20/90 | LN | HD | − | 46,XX[1] |
| 922401 | 04/23/92 | LN | HD | − | 46,XY[20] |
| 9/100 = 9% | |||||
| 932553 | 05/17/93 | LN | HD | − | 46,XY[20] |
| 871261 | 03/11/87 | LN | Follicular mixed cell lymphoma (B cell) | − | 46,XX,t(14; 18)(q32;q21)[2]/46,XX[1] |
| 930808 | 10/14/92 | LN | Follicular mixed cell lymphoma (B cell) | − | 46,XX,inv(9)(p24q32),t(14; 18)(q32;q21)[4]/46,XX[10] |
| 7/100 = 7% | |||||
| 921332† | 12/10/91 | LN | Diffuse LCL (B cell) | − | 87,XX,−Yx2,−1,dup(1)(q21q25),del(2)(p23),+del(2) (p23),−4,−6,+7x2,−9,−11,−17[10]/46,XY[3] |
| 4/100 = 4% |
The number of interphase nuclei exhibiting paired or fused green and red signals (numerator), the total number of nuclei examined (denominator), and the percentage of nuclei apparently positive for the t(2; 5) are shown for those cases in which 150 or fewer nuclei were analyzed.
In initial cytogenetic characterization, case no. 921332 was thought to contain the t(2; 5). The nomenclature shown is a revision following reexamination of the sample subsequent to NPM-ALK FISH analysis.