In Vivo and In Vitro Colony Forming Activity of bcl-2 Knockout Hematopoietic Stem Cells
| . | HPP-CFU* . | CFU-C† . | CFU-S‡ . |
|---|---|---|---|
| . | /100 Cells . | /100 Cells . | /1,000 Cells . |
| bcl-2−/− | 12.5 ± 1.5 | 16.2 ± 7.6 | 4.6 ± 1.3† |
| bcl-2+/− | 14.5 ± 2.5 | 19.0 ± 1.1 | 7.7 ± 1.7 |
| . | HPP-CFU* . | CFU-C† . | CFU-S‡ . |
|---|---|---|---|
| . | /100 Cells . | /100 Cells . | /1,000 Cells . |
| bcl-2−/− | 12.5 ± 1.5 | 16.2 ± 7.6 | 4.6 ± 1.3† |
| bcl-2+/− | 14.5 ± 2.5 | 19.0 ± 1.1 | 7.7 ± 1.7 |
The c-kit+ Sca-1+ Lin− cells from BM were sorted on the basis of staining analysis. In vitro colony assays were performed. Mean ± SD for n = 6.
Numbers of colonies in the presence of IL-1, IL-3, M-CSF, GM-CSF, and SCF(KL) at day 14.
Number of colonies in the presence of IL-3 and Epo at day 14.
Cells were injected into irradiated B6 mice intravenously and the number of spleen colonies were counted at day 12.
ρ P < .01.