Results of Immunophenotypic and Cytogenetic Analysis
| Cell Line . | Immunophenotype (%) . | Karyotype* . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| . | Tdt . | CD19 . | CD10† . | Smlg . | cμ . | FMC7 . | HLA-DR . | CD24 . | . |
| NALM-6 | 40 | 45‡ | 70 | 1 | 38 | 1 | 70 | 0 | 46, XY, t(5; 12)(q33; p12) [10] |
| PBE-1 | 40 | 32‡ | 48 | 1 | 60 | 0 | 83 | 53 | 46, XY, t(5; 12)(q33; p12) [21] |
| 47, XY, t(1; 10)(q21; q22), t(5; 12)(q33; p12), + 13 [5] | |||||||||
| LR-10.6 | 90 | 99 | 99 | 14 | 100 | 1 | 100 | 9 | 46, XY, t(5; 12)(q33; p12) [20] |
| Cell Line . | Immunophenotype (%) . | Karyotype* . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| . | Tdt . | CD19 . | CD10† . | Smlg . | cμ . | FMC7 . | HLA-DR . | CD24 . | . |
| NALM-6 | 40 | 45‡ | 70 | 1 | 38 | 1 | 70 | 0 | 46, XY, t(5; 12)(q33; p12) [10] |
| PBE-1 | 40 | 32‡ | 48 | 1 | 60 | 0 | 83 | 53 | 46, XY, t(5; 12)(q33; p12) [21] |
| 47, XY, t(1; 10)(q21; q22), t(5; 12)(q33; p12), + 13 [5] | |||||||||
| LR-10.6 | 90 | 99 | 99 | 14 | 100 | 1 | 100 | 9 | 46, XY, t(5; 12)(q33; p12) [20] |
T-cell markers CD2, CD4, and CD8, myeloid markers CD13 and CD33, and megakaryocytic marker CD41 were consistently negative in all three cell lines.
Abbreviations: Tdt, terminal deoxynucleotidyl transferase; Smlg, surface membrane Ig; cμ, cytoplasmic μ chain.
In square brackets: number of cells analyzed.
Combined CD19/CD10 staining.
Weak staining.