Modified Peptide Sequence Determined by N-Terminal Sequencing
| Cycle . | PTH-Amino Acid (pmol)* . | Amino Acid Sequence . |
|---|---|---|
| . | . | Deduced From cDNA† . |
| 1 | Gly (28) | Gly697 |
| 2 | Pro (18) | Pro698 |
| 3 | Gly (53) | Gly699 |
| 4 | His (11) | His700 |
| 5 | Pro (55) | Pro701 |
| 6 | Pro (12) | Pro702 |
| 7 | ‡ | Leu703 |
| 8 | Pro (6) | Pro704 |
| 9 | Asp (11) | Asp705 |
| 10 | ‡ | Lys706 |
| Cycle . | PTH-Amino Acid (pmol)* . | Amino Acid Sequence . |
|---|---|---|
| . | . | Deduced From cDNA† . |
| 1 | Gly (28) | Gly697 |
| 2 | Pro (18) | Pro698 |
| 3 | Gly (53) | Gly699 |
| 4 | His (11) | His700 |
| 5 | Pro (55) | Pro701 |
| 6 | Pro (12) | Pro702 |
| 7 | ‡ | Leu703 |
| 8 | Pro (6) | Pro704 |
| 9 | Asp (11) | Asp705 |
| 10 | ‡ | Lys706 |
From the measured radioactivity, 71 pmol of radioactive pepide was applied to the sequencer.
The amino acid sequence of the isolated peptide determined by N-terminal sequencing is compared with the cDNA deduced protein sequence from Livi et al.27 A second peptide, Ile-Ser-Gly-Try-Gly-Asn, was also present and was identified as derived from bovine pancreatic trypsin.30 31
No PTH derivative was detected at ≥5 pmol.