Table 1. RTA-Induced Cytotoxicity Assay... | American Society of Hematology

Table 1.

RTA-Induced Cytotoxicity

Assay	Treatment	nmol/L	Hours Posttreatment
			5 h	24 h	48 h	72 h	96 h
Protein synthesis^*	dgRTA	10	99.7 ± 9.8	95.6 ± 7.3	94.1 ± 6.6^†	ND	ND
[³H] leucine incorporation (n = 16)	dgRTA	31.6	96.5 ± 7.8	93.1 ± 8.5^‡	93.1 ± 8.5^‡	ND	ND
	dgRTA	100	100.4 ± 11.6	92.6 ± 12.7^‡	85.0 ± 12.1^‡	ND	ND
	rRTA	100	98.7 ± 12.9	89.9 ± 11.5^‡	87.1 ± 14.5^‡	ND	ND
	rRTA-E177D	100	96.8 ± 11.0	96.1 ± 7.4	97.3 ± 5.8	ND	ND
	Ricin	10	1.5 ± 0.3^‡	ND	ND	ND	ND
Metabolic activity	dgRTA	10	ND	105.6 ± 4.4^†	95.8 ± 4.8	102.0 ± 4.8	107.8 ± 6.3^†
Alamar Blue (n = 9)	dgRTA	31.6	ND	113.2 ± 1.8^‡	88.7 ± 4.5^‡	101.5 ± 4.1	104.3 ± 8.2
	dgRTA	100	ND	108.7 ± 6.2^‡	80.8 ± 7.7^‡	82.3 ± 4.6^‡	83.9 ± 7.8^‡
	rRTA	100	ND	106.4 ± 4.7^†	72.8 ± 5.6^‡	74.4 ± 5.5^‡	72.7 ± 5.3^‡
	rRTA-E177D	100	ND	107.6 ± 6.0^‡	105.2 ± 4.2	107.4 ± 6.8^‡	109.4 ± 10.2
	Ricin	10	ND	18.6 ± 2.5^‡	3.2 ± 0.4^‡	0.5 ± 0.2^‡	0.0 ± 0.0^‡
Cell density	dgRTA	10	ND	100.0 ± 19.1	93.0 ± 12.8	101.5 ± 15.1	92.7 ± 3.7
Trypan blue dye exclusion (n = 3)	dgRTA	31.6	ND	100.0 ± 11.8	97.7 ± 23.5	87.7 ± 9.9	90.0 ± 6.1^†
	dgRTA	100	ND	95.8 ± 0.8	86.9 ± 8.4	79.5 ± 9.4^†	77.6 ± 2.9^‡
	rRTA	100	ND	103.0 ± 14.7	98.8 ± 8.9	82.4 ± 7.6^†	78.4 ± 2.2^‡
	rRTA-E177D	100	ND	102.1 ± 8.8	117.5 ± 9.7	104.9 ± 4.8	109.9 ± 8.2^†
	Ricin	10	ND	ND	ND	ND	ND

Assay	Treatment	nmol/L	Hours Posttreatment
			5 h	24 h	48 h	72 h	96 h
Protein synthesis^*	dgRTA	10	99.7 ± 9.8	95.6 ± 7.3	94.1 ± 6.6^†	ND	ND
[³H] leucine incorporation (n = 16)	dgRTA	31.6	96.5 ± 7.8	93.1 ± 8.5^‡	93.1 ± 8.5^‡	ND	ND
	dgRTA	100	100.4 ± 11.6	92.6 ± 12.7^‡	85.0 ± 12.1^‡	ND	ND
	rRTA	100	98.7 ± 12.9	89.9 ± 11.5^‡	87.1 ± 14.5^‡	ND	ND
	rRTA-E177D	100	96.8 ± 11.0	96.1 ± 7.4	97.3 ± 5.8	ND	ND
	Ricin	10	1.5 ± 0.3^‡	ND	ND	ND	ND
Metabolic activity	dgRTA	10	ND	105.6 ± 4.4^†	95.8 ± 4.8	102.0 ± 4.8	107.8 ± 6.3^†
Alamar Blue (n = 9)	dgRTA	31.6	ND	113.2 ± 1.8^‡	88.7 ± 4.5^‡	101.5 ± 4.1	104.3 ± 8.2
	dgRTA	100	ND	108.7 ± 6.2^‡	80.8 ± 7.7^‡	82.3 ± 4.6^‡	83.9 ± 7.8^‡
	rRTA	100	ND	106.4 ± 4.7^†	72.8 ± 5.6^‡	74.4 ± 5.5^‡	72.7 ± 5.3^‡
	rRTA-E177D	100	ND	107.6 ± 6.0^‡	105.2 ± 4.2	107.4 ± 6.8^‡	109.4 ± 10.2
	Ricin	10	ND	18.6 ± 2.5^‡	3.2 ± 0.4^‡	0.5 ± 0.2^‡	0.0 ± 0.0^‡
Cell density	dgRTA	10	ND	100.0 ± 19.1	93.0 ± 12.8	101.5 ± 15.1	92.7 ± 3.7
Trypan blue dye exclusion (n = 3)	dgRTA	31.6	ND	100.0 ± 11.8	97.7 ± 23.5	87.7 ± 9.9	90.0 ± 6.1^†
	dgRTA	100	ND	95.8 ± 0.8	86.9 ± 8.4	79.5 ± 9.4^†	77.6 ± 2.9^‡
	rRTA	100	ND	103.0 ± 14.7	98.8 ± 8.9	82.4 ± 7.6^†	78.4 ± 2.2^‡
	rRTA-E177D	100	ND	102.1 ± 8.8	117.5 ± 9.7	104.9 ± 4.8	109.9 ± 8.2^†
	Ricin	10	ND	ND	ND	ND	ND

Data are presented as the mean percentage of time-matched untreated controls ± SD. Abbreviation: ND, not determined.

Data are combined from two separate experiments.

P < .05 compared with time-matched untreated controls.

P < .01 compared with time-matched untreated controls.