Table 1. Demonstration That Cells... | American Society of Hematology

Table 1.

Demonstration That Cells Responding to HA Are B Cells

Mitogen	Cell Proliferation (CPM ± SE)^*
	Experiment 1		Experiment 2
	Absence of PB	Presence of PB^†	C57BL/6	C3H/HeJ
None	3,041 ± 225	3,370 ± 865	5,552 ± 223	10,823 ± 466
HA	92,428 ± 510	80,958 ± 3,945	80,362 ± 1,809	111,222 ± 4,505
LPS 1 μg/mL	137,833 ± 7,909	5,283 ± 204	100,080 ± 837	22,569 ± 1,318
LPS 5 μg/mL	144,597 ± 6,893	18,491 ± 2,377	115,214 ± 8,048	37,718 ± 639
LPS 10 μg/mL	156,766 ± 3,383	42,527 ± 5,751	124,387 ± 2,694	43,423 ± 975

Mitogen	Cell Proliferation (CPM ± SE)^*
	Experiment 1		Experiment 2
	Absence of PB	Presence of PB^†	C57BL/6	C3H/HeJ
None	3,041 ± 225	3,370 ± 865	5,552 ± 223	10,823 ± 466
HA	92,428 ± 510	80,958 ± 3,945	80,362 ± 1,809	111,222 ± 4,505
LPS 1 μg/mL	137,833 ± 7,909	5,283 ± 204	100,080 ± 837	22,569 ± 1,318
LPS 5 μg/mL	144,597 ± 6,893	18,491 ± 2,377	115,214 ± 8,048	37,718 ± 639
LPS 10 μg/mL	156,766 ± 3,383	42,527 ± 5,751	124,387 ± 2,694	43,423 ± 975

Abbreviation: PB, polymixin B.

Spleen cells from C57BL/6 mice were cultured with various mitogens for 48 hours and cell proliferation was measured by ³H-Thymidine uptake assay. The results are expressed as mean counts per minute (CPM) ± SE.

†

PB was used at a concentration of 5 μg/mL.

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