Designation of YAC Clones, Chromosomal Localization, Size, and DNA Markers/Genes on Each YAC Clone
| Name . | Location . | Size (kb) . | DNA Markers . |
|---|---|---|---|
| HSC7E520 | 7q21.1 | 500 | CACNL2A, HGF, D7S581, D7S802, D7S849, D7S2275E |
| HSC7E590 | 7q21.1-q21.2 | 460 | D7S166, D7S195, D7S260, D7S524, D7S644, D7S2537 |
| HSC7E481 | 7q21.1-q21.3 | 900 | PGY1, PGY3, D7S269, D7S575, D7S1642, D7S1646, D7S2621, D7S2623, D7S2624, D7S2626, D7S2628, D7S2786, Cdy11e05 |
| HSC7E502 | 7q21.1-q21.3 | 600 | D7S183, D7S492, D7S569, D7S570, D7S577 |
| HSC7E465 | 7q21.3 | 700 | CALCR, D7S15, D7S652, D7S657, D7S2430, HSC7E1275RV |
| HSC7E783 | 7q21.3-q22.1 | 650 | ASNS, D7S82, D7S554, HSC7E1131RV, UT901 |
| HSC7E911 | 7q22 | 580 | D7S651, NIB217, 932D8.1, E911.1 |
| HSC7E808 | 7q22 | 470 | D7S647, D7S2498, D7S2832, AFMa163ya9, E808.3, CYP3A4 |
| HSC7E914 | 7q22 | 340 | D7S647, D7S2832, AFMa163ya9, CYP3A4 |
| HSC7E737 | 7q22 | 250 | PLANHI, D7S477, UT682 |
| HSC7E791 | 7q22 | 250 | CUTL1, D7S515, D7S666, D7S740, D7S2433, D7S2536 |
| HSC7E329 | 7q22 | 380 | D7S2448, APCR.HSC7E452, APCR.HSC7E570, 942E8.18 |
| HSC7E316 | 7q22 | 440 | APCR.HSC7E452, 942E8.18 |
| HSC7E441 | 7q22 | 480 | D7S76, D7S238, D7S561, D7S658, D7S796, D7S818, D7S2504 |
| HSC7E485 | 7q22 | 540 | D7S76, D7S238, D7S561, D7S562, D7S563, D7S796, D7S818 |
| HSC7E506 | 7q22 | 520 | D7S240, D7S561, D7S562, D7S563, D7S572, D7S579, D7S584, D7S2509 |
| HSC7E572 | 7q22.3-q31.1 | 400 | D7S1493, D7S1799, D7S2494 |
| HSC7E161 | 7q22.3-q31.1 | 450 | PRKAR2B, DRA, D7S496, D7S2317, D7S2459, Bdy96E06 |
| HSC7E132 | 7q22.3-q31.1 | 650 | D7S13, D7S1763, Bdy31e11 |
| HSC7E4 | 7q31.2-q31.3 | 600 | CAPZA2, MET, D7S567, D7S568, D7S1764, D7S2460 |
| HSC7E222 | 7q31.3-q32.1 | 580 | D7S480, D7S650, D7S685, D7S2821, Cdy0rd04 |
| HSC7E1289 | 7q32.1 | 1800 | GRM8, D7S514, D7S680, D7S686, D7S1498, D7S1801, D7S1822, D7S1873, D7S1874, D7S2244 |
| HSC7E526 | 7q36 | 560 | D7S68, D7S593, D7S594 |
| Name . | Location . | Size (kb) . | DNA Markers . |
|---|---|---|---|
| HSC7E520 | 7q21.1 | 500 | CACNL2A, HGF, D7S581, D7S802, D7S849, D7S2275E |
| HSC7E590 | 7q21.1-q21.2 | 460 | D7S166, D7S195, D7S260, D7S524, D7S644, D7S2537 |
| HSC7E481 | 7q21.1-q21.3 | 900 | PGY1, PGY3, D7S269, D7S575, D7S1642, D7S1646, D7S2621, D7S2623, D7S2624, D7S2626, D7S2628, D7S2786, Cdy11e05 |
| HSC7E502 | 7q21.1-q21.3 | 600 | D7S183, D7S492, D7S569, D7S570, D7S577 |
| HSC7E465 | 7q21.3 | 700 | CALCR, D7S15, D7S652, D7S657, D7S2430, HSC7E1275RV |
| HSC7E783 | 7q21.3-q22.1 | 650 | ASNS, D7S82, D7S554, HSC7E1131RV, UT901 |
| HSC7E911 | 7q22 | 580 | D7S651, NIB217, 932D8.1, E911.1 |
| HSC7E808 | 7q22 | 470 | D7S647, D7S2498, D7S2832, AFMa163ya9, E808.3, CYP3A4 |
| HSC7E914 | 7q22 | 340 | D7S647, D7S2832, AFMa163ya9, CYP3A4 |
| HSC7E737 | 7q22 | 250 | PLANHI, D7S477, UT682 |
| HSC7E791 | 7q22 | 250 | CUTL1, D7S515, D7S666, D7S740, D7S2433, D7S2536 |
| HSC7E329 | 7q22 | 380 | D7S2448, APCR.HSC7E452, APCR.HSC7E570, 942E8.18 |
| HSC7E316 | 7q22 | 440 | APCR.HSC7E452, 942E8.18 |
| HSC7E441 | 7q22 | 480 | D7S76, D7S238, D7S561, D7S658, D7S796, D7S818, D7S2504 |
| HSC7E485 | 7q22 | 540 | D7S76, D7S238, D7S561, D7S562, D7S563, D7S796, D7S818 |
| HSC7E506 | 7q22 | 520 | D7S240, D7S561, D7S562, D7S563, D7S572, D7S579, D7S584, D7S2509 |
| HSC7E572 | 7q22.3-q31.1 | 400 | D7S1493, D7S1799, D7S2494 |
| HSC7E161 | 7q22.3-q31.1 | 450 | PRKAR2B, DRA, D7S496, D7S2317, D7S2459, Bdy96E06 |
| HSC7E132 | 7q22.3-q31.1 | 650 | D7S13, D7S1763, Bdy31e11 |
| HSC7E4 | 7q31.2-q31.3 | 600 | CAPZA2, MET, D7S567, D7S568, D7S1764, D7S2460 |
| HSC7E222 | 7q31.3-q32.1 | 580 | D7S480, D7S650, D7S685, D7S2821, Cdy0rd04 |
| HSC7E1289 | 7q32.1 | 1800 | GRM8, D7S514, D7S680, D7S686, D7S1498, D7S1801, D7S1822, D7S1873, D7S1874, D7S2244 |
| HSC7E526 | 7q36 | 560 | D7S68, D7S593, D7S594 |
The region between YAC clones HSC7E808 to HSC7E572 represents approximately 5 to 6 Mb of DNA and this region is covered completely by an overlapping set of YAC clones.17 HSC7E914, HSC7E737, HSC7E791, HSC7E329, HSC7E316, HSC7E441, HSC7E485, and HSC7E506 are representative clones from this contig that were chosen to be uniformly spaced throughout the region. The genes encoding EPO and ACHE are located between clones HSC7E911 and HSC7E808.20 To mark the end of the chromosome, we used clone HSC7E526, which begins approximately 100 kb from the end of the chromosome and extends 560 kb towards the centromere.20