Effects of Sugars on Calcium Responses in Namalwa Cells
| Experiment . | Sugars . | MFI (arbitrary units) . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | . | Basal Level . | Anti-B4–bR . | Anti-B4–bR + GAM . | . | . | ||
| . | . | . | . | . | . | . | . | . |
| 1 | No sugar | 215 | 371 | 522 | ||||
| Lactose (50 mmol/L) | 225 | 328 | (−34%)* | 521 | (−4%) | |||
| Maltose (50 mmol/L) | 229 | 345 | (−26%) | 548 | (+4%) | |||
| 2 | No sugar | 230 | 412 | 696 | ||||
| Lactose (50 mmol/L) | 222 | 324 | (−44%) | 637 | (−11%) | |||
| Maltose (50 mmol/L) | 212 | 353 | (−23%) | 678 | (0) | |||
| 3 | No sugar | 223 | 385 | 703 | ||||
| Lactose (50 mmol/L) | 224 | 314 | (−44%) | 640 | (−13%) | |||
| Galactose (50 mmol/L) | 212 | 366 | (−5%) | 720 | (+6%) | |||
| Experiment . | Sugars . | MFI (arbitrary units) . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | . | Basal Level . | Anti-B4–bR . | Anti-B4–bR + GAM . | . | . | ||
| . | . | . | . | . | . | . | . | . |
| 1 | No sugar | 215 | 371 | 522 | ||||
| Lactose (50 mmol/L) | 225 | 328 | (−34%)* | 521 | (−4%) | |||
| Maltose (50 mmol/L) | 229 | 345 | (−26%) | 548 | (+4%) | |||
| 2 | No sugar | 230 | 412 | 696 | ||||
| Lactose (50 mmol/L) | 222 | 324 | (−44%) | 637 | (−11%) | |||
| Maltose (50 mmol/L) | 212 | 353 | (−23%) | 678 | (0) | |||
| 3 | No sugar | 223 | 385 | 703 | ||||
| Lactose (50 mmol/L) | 224 | 314 | (−44%) | 640 | (−13%) | |||
| Galactose (50 mmol/L) | 212 | 366 | (−5%) | 720 | (+6%) | |||
Data are expressed as the peak values of the MFI of Fluo-3-loaded cells before (basal level) and after addition of anti-B4–bR (anti-B4–bR) followed by GAM (anti-B4–bR + GAM).
Percent change of MFI by sugar compared with control without sugar, after substraction of corresponding basal levels.