Effects of Multiple Cytokines on the Reconstituting Ability of Cultured Cells
| Cytokines in Culture . | No. of Cells Injected . | No. of CFU-C Injected . | % Ly5.1 (donor) PB Cells5-150 . | |
|---|---|---|---|---|
| . | . | . | 2 mo . | 6 mo . |
| Experiment 1 | ||||
| Fresh marrow | 100 | 48 | 47.1 ± 22.8 | 54.6 ± 29.8 |
| FL,IL-6,IL-11,Ep | 2.7 × 104 | 4,500 | 58.7 ± 19.1 | 42.0 ± 18.7 |
| KL,IL-6,IL-11,Ep | 3.3 × 105 | 30,000 | 72.9 ± 11.2 | 48.3 ± 26.2 |
| Experiment 2 | ||||
| Fresh marrow | 500 | 240 | 71.3 ± 17.8 | 65.1 ± 28.3 |
| KL,IL-6,IL-11,Ep | 8.0 × 105 | 182,000 | 69.5 ± 17.5 | 63.5 ± 30.6 |
| FL,KL,IL-6,IL-11,Ep | 1.5 × 106 | 281,000 | 80.5 ± 16.2 | 73.8 ± 22.6 |
| IL-3,KL,IL-6,IL-11,Ep | 3.3 × 106 | 35,000 | 19.3 ± 11.1 | 14.5 ± 11.5 |
| Cytokines in Culture . | No. of Cells Injected . | No. of CFU-C Injected . | % Ly5.1 (donor) PB Cells5-150 . | |
|---|---|---|---|---|
| . | . | . | 2 mo . | 6 mo . |
| Experiment 1 | ||||
| Fresh marrow | 100 | 48 | 47.1 ± 22.8 | 54.6 ± 29.8 |
| FL,IL-6,IL-11,Ep | 2.7 × 104 | 4,500 | 58.7 ± 19.1 | 42.0 ± 18.7 |
| KL,IL-6,IL-11,Ep | 3.3 × 105 | 30,000 | 72.9 ± 11.2 | 48.3 ± 26.2 |
| Experiment 2 | ||||
| Fresh marrow | 500 | 240 | 71.3 ± 17.8 | 65.1 ± 28.3 |
| KL,IL-6,IL-11,Ep | 8.0 × 105 | 182,000 | 69.5 ± 17.5 | 63.5 ± 30.6 |
| FL,KL,IL-6,IL-11,Ep | 1.5 × 106 | 281,000 | 80.5 ± 16.2 | 73.8 ± 22.6 |
| IL-3,KL,IL-6,IL-11,Ep | 3.3 × 106 | 35,000 | 19.3 ± 11.1 | 14.5 ± 11.5 |
Irradiated recipients (5 mice per group) were transplanted with 100 (experiment 1) or 500 (experiment 2) freshly prepared Lin−Sca-1+c-kit+ cells or with the whole fractions of day 7 cultured cells that had been initiated with 100 or 500 Lin−Sca-1+c-kit+ cells.
Mean ± SD % donor-derived nucleated peripheral blood cells identified by anti-Ly5.1. Background (peripheral blood cells of nontransplanted female mice) staining was <1.0%.