Reconstituting Ability of Fresh Bone Marrow and Day 7 or Day 21 Cultured Cells
| Cytokines . | Days of Incubation . | No. of Cells Injected . | No. of CFU-C Injected . | % Ly5.1 (donor) PB Cells4-150 . | |
|---|---|---|---|---|---|
| . | . | . | . | 2 mo . | 4 mo . |
| Fresh marrow | 100 | 42 | 51.3 ± 14.1 | 52.3 ± 20.8 | |
| FL, IL-11 | 7 | 1.6 × 104 | ND | 50.5 ± 8.3 | 40.1 ± 8.60 |
| KL, IL-11 | 7 | 2.0 × 105 | ND | 63.4 ± 12.2 | 44.7 ± 16.9 |
| FL, IL-11 | 21 | 3.4 × 107 | 34,100 | 47.3 ± 8.5 | 36.2 ± 16.8 |
| KL, IL-11 | 21 | 3.2 × 107 | 9,200 | 16.3 ± 2.5 | 11.7 ± 2.2 |
| Cytokines . | Days of Incubation . | No. of Cells Injected . | No. of CFU-C Injected . | % Ly5.1 (donor) PB Cells4-150 . | |
|---|---|---|---|---|---|
| . | . | . | . | 2 mo . | 4 mo . |
| Fresh marrow | 100 | 42 | 51.3 ± 14.1 | 52.3 ± 20.8 | |
| FL, IL-11 | 7 | 1.6 × 104 | ND | 50.5 ± 8.3 | 40.1 ± 8.60 |
| KL, IL-11 | 7 | 2.0 × 105 | ND | 63.4 ± 12.2 | 44.7 ± 16.9 |
| FL, IL-11 | 21 | 3.4 × 107 | 34,100 | 47.3 ± 8.5 | 36.2 ± 16.8 |
| KL, IL-11 | 21 | 3.2 × 107 | 9,200 | 16.3 ± 2.5 | 11.7 ± 2.2 |
Irradiated recipients (5 mice per group) were transplanted with 100 fresh Lin−Sca-1+c-kit+ cells or with the whole fractions of day 7 or day 21 cultured cells that had been initiated with 100 Lin−Sca-1+c-kit+ cells.
Mean ± SD % donor-derived nucleated peripheral blood cells identified by anti-Ly5.1. Background (peripheral blood cells of nontransplanted female mice) staining was <1.0%.